Thermodynamic Analysis of Autonomous Parallel Beta-sheet Formation in Water

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2006 Jun 1

PMID 16734453

Citations 12

Authors

John D Fisk

Margaret A Schmitt

Samuel H Gellman

Affiliations

Soon will be listed here.

Abstract

We report the first thermodynamic analysis of parallel beta-sheet formation in a model system that folds in aqueous solution. NMR chemical shifts were used to determine beta-sheet population, and van't Hoff anaysis provided thermodynamic parameters. Our approach relies upon the d-prolyl-1,1-dimethyl-1,2-diaminoethane unit to promote parallel beta-sheet formation between attached peptide strands. The development of a macrocyclic reference molecule to provide chemical shift data for the fully folded state was crucial to the quantitative anaylsis.

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