Cigarette Smoke Affects Apoptosis in Rat Tongue Mucosa: Role of Bcl-2 Gene Family

Overview

Journal J Mol Histol

Specialty Biochemistry

Date 2006 May 31

PMID 16733793

Citations 14

Authors

Gerson F Assis

Daniele S Ceolin

Mariangela E A Marques

Daisy M F Salvadori

Daniel A Ribeiro

Affiliations

Soon will be listed here.

Abstract

While it has been clearly demonstrated that smoking is the most significant exogenous factor involved in oral carcinogenesis, little is known about the global molecular and cellular changes that occur prior to the appearance of clinically detectable symptoms. Thus, the aim of this study was to investigate the expressivity of bcl-2, bax and PCNA in the rat tongue mucosa exposed to cigarette smoke by means of immunohistochemistry. A total of twelve male Wistar rats were distributed into 2 groups: negative control and experimental group exposed to cigarette smoke during 75 days. After experimental period, no histopathological changes in the tongue mucosa were detected in the negative control and the experimental group. On the other hand, an overexpression of bcl-2 was detected (p < 0.01) throughout all layers of the epithelium, whereas bax did not show significant differences (p > 0.05). Also, the labeling index for bcl-2 and bax showed an increase 75 days after cigarette exposure (p < 0.01). PCNA-labeling index did not show remarkable changes between groups. Taken together, our results show that bcl-2 is overexpressed in the rat tongue keratinocytes after cigarette smoke exposure.

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References

Jordan R, Catzavelos G, Barrett A, Speight P . Differential expression of bcl-2 and bax in squamous cell carcinomas of the oral cavity. Eur J Cancer B Oral Oncol. 1996; 32B(6):394-400. DOI: 10.1016/s0964-1955(96)00033-4. View

Linden M, Torres F, Kubus J, Zarbo R . Clinical application of morphologic and immunocytochemical assessments of cell proliferation. Am J Clin Pathol. 1992; 97(5 Suppl 1):S4-13. View

Armstrong J . Mitochondria: a target for cancer therapy. Br J Pharmacol. 2005; 147(3):239-48. PMC: 1751297. DOI: 10.1038/sj.bjp.0706556. View

Akao Y, Otsuki Y, Kataoka S, Ito Y, Tsujimoto Y . Multiple subcellular localization of bcl-2: detection in nuclear outer membrane, endoplasmic reticulum membrane, and mitochondrial membranes. Cancer Res. 1994; 54(9):2468-71. View

Loro L, Vintermyr O, Johannessen A . Apoptosis in normal and diseased oral tissues. Oral Dis. 2005; 11(5):274-87. DOI: 10.1111/j.1601-0825.2005.01117.x. View

Xi S, Grandis J . Gene therapy for the treatment of oral squamous cell carcinoma. J Dent Res. 2003; 82(1):11-6. DOI: 10.1177/154405910308200104. View

Krajewski S, Krajewska M, Shabaik A, Miyashita T, Wang H, Reed J . Immunohistochemical determination of in vivo distribution of Bax, a dominant inhibitor of Bcl-2. Am J Pathol. 1994; 145(6):1323-36. PMC: 1887502. View

Okazaki Y, Tanaka Y, Tonogi M, Yamane G . Investigation of environmental factors for diagnosing malignant potential in oral epithelial dysplasia. Oral Oncol. 2002; 38(6):562-73. DOI: 10.1016/s1368-8375(01)00119-1. View

Magrath I, LITVAK J . Cancer in developing countries: opportunity and challenge. J Natl Cancer Inst. 1993; 85(11):862-74. DOI: 10.1093/jnci/85.11.862. View

10.

Ribeiro D, Salvadori D, Marques M . Abnormal expression of bcl-2 and bax in rat tongue mucosa during the development of squamous cell carcinoma induced by 4-nitroquinoline 1-oxide. Int J Exp Pathol. 2005; 86(6):375-81. PMC: 2517447. DOI: 10.1111/j.0959-9673.2005.00444.x. View

11.

Polverini P, Nor J . Apoptosis and predisposition to oral cancer. Crit Rev Oral Biol Med. 2000; 10(2):139-52. DOI: 10.1177/10454411990100020201. View

12.

Liu W, Bulgaru A, Haigentz M, Stein C, Perez-Soler R, Mani S . The BCL2-family of protein ligands as cancer drugs: the next generation of therapeutics. Curr Med Chem Anticancer Agents. 2003; 3(3):217-23. DOI: 10.2174/1568011033482459. View

13.

Bagchi M, Balmoori J, Bagchi D, Ray S, Kuszynski C, Stohs S . Smokeless tobacco, oxidative stress, apoptosis, and antioxidants in human oral keratinocytes. Free Radic Biol Med. 1999; 26(7-8):992-1000. DOI: 10.1016/s0891-5849(98)00286-x. View

14.

KERR J, Wyllie A, Currie A . Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer. 1972; 26(4):239-57. PMC: 2008650. DOI: 10.1038/bjc.1972.33. View

15.

Schwartz J, Muscat J, Baker V, Larios E, Stephenson G, Guo W . Oral cytology assessment by flow cytometry of DNA adducts, aneuploidy, proliferation and apoptosis shows differences between smokers and non-smokers. Oral Oncol. 2003; 39(8):842-54. DOI: 10.1016/s1368-8375(03)00107-6. View

16.

Loro L, Vintermyr O, Johannessen A . Cell death regulation in oral squamous cell carcinoma: methodological considerations and clinical significance. J Oral Pathol Med. 2003; 32(3):125-38. DOI: 10.1034/j.1600-0714.2003.00052.x. View

17.

Hahn M, Hagedorn G, Kuhlisch E, Schackert H, Eckelt U . Genetic polymorphisms of drug-metabolizing enzymes and susceptibility to oral cavity cancer. Oral Oncol. 2002; 38(5):486-90. DOI: 10.1016/s1368-8375(01)00086-0. View

18.

Simani A, Inoue S, Hogg J . Penetration of the respiratory epithelium of guinea pigs following exposure to cigarette smoke. Lab Invest. 1974; 31(1):75-81. View

19.

Lee H, Guo H, Lee S, Jeon B, Jun C, Lee S . Effects of nicotine on proliferation, cell cycle, and differentiation in immortalized and malignant oral keratinocytes. J Oral Pathol Med. 2005; 34(7):436-43. DOI: 10.1111/j.1600-0714.2005.00342.x. View

20.

Reed J . Bcl-2 and the regulation of programmed cell death. J Cell Biol. 1994; 124(1-2):1-6. PMC: 2119888. DOI: 10.1083/jcb.124.1.1. View