Actin Polymerisation at the Cytoplasmic Face of Eukaryotic Nuclei

Overview

Journal BMC Cell Biol

Publisher Biomed Central

Specialty Cell Biology

Date 2006 May 25

PMID 16719903

Citations 17

Authors

Sylvia Munter

Jost Enninga

Rafael Vazquez-Martinez

Erwan Delbarre

Brigitte David-Watine

Ulf Nehrbass

Spencer L Shorte

Affiliations

Soon will be listed here.

Abstract

Background: There exists abundant molecular and ultra-structural evidence to suggest that cytoplasmic actin can physically interact with the nuclear envelope (NE) membrane system. However, this interaction has yet to be characterised in living interphase cells.

Results: Using a fluorescent conjugate of the actin binding drug cytochalasin D (CD-BODIPY) we provide evidence that polymerising actin accumulates in vicinity to the NE. In addition, both transiently expressed fluorescent actin and cytoplasmic micro-injection of fluorescent actin resulted in accumulation of actin at the NE-membrane. Consistent with the idea that the cytoplasmic phase of NE-membranes can support this novel pool of perinuclear actin polymerisation we show that isolated, intact, differentiated primary hepatocyte nuclei support actin polymerisation in vitro. Further this phenomenon was inhibited by treatments hindering steric access to outer-nuclear-membrane proteins (e.g. wheat germ agglutinin, anti-nesprin and anti-nucleoporin antibodies).

Conclusion: We conclude that actin polymerisation occurs around interphase nuclei of living cells at the cytoplasmic phase of NE-membranes.

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