Osmotic Signal Transduction to ProU is Independent of DNA Supercoiling in Escherichia Coli

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Jan 1

PMID 1670937

Citations 11

Authors

R M Ramirez

M VILLAREJO

Affiliations

Soon will be listed here.

Abstract

proU expression has been proposed to form part of a general stress response that is regulated by increased negative DNA supercoiling brought about by environmental signals such as osmotic or anaerobic stress (N. Ni Bhriain, C. J. Dorman, and C. F. Higgins, Mol. Microbiol. 3:933-944, 1989). However, we find that although proU-containing plasmids derived from cells grown in media of elevated osmolarity were more supercoiled than plasmids from cells grown in standard media, they did not activate proU expression in vitro. The gyrA96 mutation and anaerobic conditions are known to affect DNA supercoiling but did not alter proU expression. Finally, the gyrase inhibitors coumermycin and novobiocin did not reduce in vitro proU expression. Therefore, this evidence rules out regulation by changes in DNA superhelicity for proU in Escherichia coli.

Citing Articles

The environmentally-regulated interplay between local three-dimensional chromatin organisation and transcription of proVWX in E. coli.

Rashid F, Cremazy F, Hofmann A, Forrest D, Grainger D, Heermann D Nat Commun. 2023; 14(1):7478.

PMID: 37978176 PMC: 10656529. DOI: 10.1038/s41467-023-43322-y.

How is osmotic regulation of transcription of the Escherichia coli proU operon achieved? A review and a model.

Gowrishankar J, Manna D Genetica. 1996; 97(3):363-78.

PMID: 9081863 DOI: 10.1007/BF00055322.

The Escherichia coli nucleoid protein H-NS functions directly as a transcriptional repressor.

Ueguchi C, Mizuno T EMBO J. 1993; 12(3):1039-46.

PMID: 8458322 PMC: 413305. DOI: 10.1002/j.1460-2075.1993.tb05745.x.

Osmotic repression of anaerobic metabolic systems in Escherichia coli.

Gouesbet G, Abaibou H, Wu L, Mandrand-Berthelot M, Blanco C J Bacteriol. 1993; 175(1):214-21.

PMID: 8416896 PMC: 196116. DOI: 10.1128/jb.175.1.214-221.1993.

The accumulation of glutamate is necessary for optimal growth of Salmonella typhimurium in media of high osmolality but not induction of the proU operon.

Csonka L, Ikeda T, Fletcher S, Kustu S J Bacteriol. 1994; 176(20):6324-33.

PMID: 7929004 PMC: 196974. DOI: 10.1128/jb.176.20.6324-6333.1994.

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