Exonuclease Requirements for Recombination of Lambda-phage in RecD Mutants of Escherichia Coli

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2006 May 17

PMID 16702415

Citations 6

Authors

Damir dermic

Davor Zahradka

Mirjana Petranovic

Affiliations

Soon will be listed here.

Abstract

Recombination of lambda red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5'-3' exonuclease activity of RecJ and exonuclease VII is essential for lambda-recombination, whereas exonucleases of 3'-5' polarity are dispensable. The implications of the presented data on current models for recombination initiation in E. coli are discussed.

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