Differential Expression of Genes Related to HFE and Iron Status in Mouse Duodenal Epithelium

Overview

Journal Mamm Genome

Specialty Genetics

Date 2006 May 12

PMID 16688533

Citations 2

Authors

Emmanuelle Abgueguen

Bertrand Toutain

Helene Bedrine

Celine Chicault

Magali Orhant

Marc Aubry

Annabelle Monnier

Stephanie Mottier

Helene Jouan

Seiamak Bahram

Jean Mosser

Patricia Fergelot

Affiliations

Soon will be listed here.

Abstract

Iron absorption, distribution, use, and storage are thought to be tightly regulated since altered iron stores may lead to cellular damage and disease. HFE, the hereditary hemochromatosis gene product, is expressed in the crypts of the duodenum, but the molecular mechanism by which it contributes to the inhibition of iron absorption is still unknown. In this study we aimed to identify transcriptional profiles in the duodenal epithelium of Hfe(-/-) mice. We used dedicated microarrays to compare gene expression among the duodenum of Hfe(-/-) mice, induced iron overload mice, and control mice. We found 151 differentially expressed genes and unknown sequences between Hfe(-/-) mice and normal littermates. Gene profiling revealed a gene subset more specific for Hfe inactivation. The functional annotation of upregulated genes highlighted that mucus production and cell maintenance may account for the influence of Hfe on epithelium integrity and luminal iron uptake.

Citing Articles

Global transcriptional response to Hfe deficiency and dietary iron overload in mouse liver and duodenum.

Rodriguez A, Luukkaala T, Fleming R, Britton R, Bacon B, Parkkila S PLoS One. 2009; 4(9):e7212.

PMID: 19787063 PMC: 2747280. DOI: 10.1371/journal.pone.0007212.

Small proline-rich proteins (SPRR) function as SH3 domain ligands, increase resistance to injury and are associated with epithelial-mesenchymal transition (EMT) in cholangiocytes.

Demetris A, Specht S, Nozaki I, Lunz 3rd J, Stolz D, Murase N J Hepatol. 2007; 48(2):276-88.

PMID: 18155796 PMC: 2263141. DOI: 10.1016/j.jhep.2007.09.019.

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