Novel Approach to Designing Primers for Identification and Distinction of the Human Pathogenic Fungi Coccidioides Immitis and Coccidioides Posadasii by PCR Amplification

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2006 May 5

PMID 16672423

Citations 14

Authors

Takashi Umeyama

Ayako Sano

Katsuhiko Kamei

Masakazu Niimi

Kazuko Nishimura

Yoshimasa Uehara

Affiliations

Soon will be listed here.

Abstract

We developed a pair of primers that specifically identifies Coccidioides species, etiologic agents of the human fungal disease coccidioidomycosis. These primers could be used for distinguishing Coccidioides immitis and Coccidioides posadasii by simply comparing the amplicon sizes on an agarose gel.

Citing Articles

Clinical Cases of Coccidioidomycosis in the Americas in the Period 1950-2021: Epidemiology Data, Diagnosis, and Treatment.

Reyes-Montes M, Leon-Garcia A, Frias-De-Leon M, Acosta-Altamirano G, Sanchez-Saavedra E, Victoriano-Pastelin I Life (Basel). 2023; 13(11).

PMID: 38004249 PMC: 10672650. DOI: 10.3390/life13112109.

Diagnosis and Treatment of Pulmonary Coccidioidomycosis and Paracoccidioidomycosis.

Pecanha-Pietrobom P, Tirado-Sanchez A, Goncalves S, Bonifaz A, Colombo A J Fungi (Basel). 2023; 9(2).

PMID: 36836333 PMC: 9959547. DOI: 10.3390/jof9020218.

Real-time PCR assay for detection and differentiation of Coccidioides immitis and Coccidioides posadasii from culture and clinical specimens.

Chaturvedi S, Victor T, Marathe A, Sidamonidze K, Crucillo K, Chaturvedi V PLoS Negl Trop Dis. 2021; 15(9):e0009765.

PMID: 34529679 PMC: 8486383. DOI: 10.1371/journal.pntd.0009765.

Design and evaluation of an AFLP molecular marker for the detection of Coccidioides spp. in biological samples.

Reyes-Montes M, Frias-De-Leon M, Victoriano-Pastelin I, Acosta-Altamirano G, Duarte-Escalante E Braz J Infect Dis. 2019; 23(5):322-330.

PMID: 31539511 PMC: 9428003. DOI: 10.1016/j.bjid.2019.08.002.

Facial rash in a renal transplant patient.

Okon E, Kim D IDCases. 2017; 9:95-96.

PMID: 28725565 PMC: 5508495. DOI: 10.1016/j.idcr.2017.05.013.

References

Fisher M, Koenig G, White T, Taylor J . Molecular and phenotypic description of Coccidioides posadasii sp. nov., previously recognized as the non-California population of Coccidioides immitis. Mycologia. 2010; 94(1):73-84. View

Johannesson H, Vidal P, Guarro J, Herr R, Cole G, Taylor J . Positive directional selection in the proline-rich antigen (PRA) gene among the human pathogenic fungi Coccidioides immitis, C. posadasii and their closest relatives. Mol Biol Evol. 2004; 21(6):1134-45. DOI: 10.1093/molbev/msh124. View

Johnson S, Simmons K, Pappagianis D . Amplification of coccidioidal DNA in clinical specimens by PCR. J Clin Microbiol. 2004; 42(5):1982-5. PMC: 404645. DOI: 10.1128/JCM.42.5.1982-1985.2004. View

Fisher M, Koenig G, White T, San-Blas G, Negroni R, Alvarez I . Biogeographic range expansion into South America by Coccidioides immitis mirrors New World patterns of human migration. Proc Natl Acad Sci U S A. 2001; 98(8):4558-62. PMC: 31873. DOI: 10.1073/pnas.071406098. View

Stockman L, Clark K, Hunt J, Roberts G . Evaluation of commercially available acridinium ester-labeled chemiluminescent DNA probes for culture identification of Blastomyces dermatitidis, Coccidioides immitis, Cryptococcus neoformans, and Histoplasma capsulatum. J Clin Microbiol. 1993; 31(4):845-50. PMC: 263575. DOI: 10.1128/jcm.31.4.845-850.1993. View

Sandhu G, Kline B, Stockman L, Roberts G . Molecular probes for diagnosis of fungal infections. J Clin Microbiol. 1995; 33(11):2913-9. PMC: 228606. DOI: 10.1128/jcm.33.11.2913-2919.1995. View

Shubitz L, Peng T, Perrill R, Simons J, Orsborn K, Galgiani J . Protection of mice against Coccidioides immitis intranasal infection by vaccination with recombinant antigen 2/PRA. Infect Immun. 2002; 70(6):3287-9. PMC: 127985. DOI: 10.1128/IAI.70.6.3287-3289.2002. View

Bialek R . Amplification of coccidioidal DNA in clinical specimens by PCR. J Clin Microbiol. 2005; 43(3):1492. PMC: 1081299. DOI: 10.1128/JCM.43.3.1492-1493.2005. View

Sano A, Miyaji M, Kamei K, Mikami Y, Nishimura K . Reexamination of Coccidioides spp. reserved in the Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, based on a multiple gene analysis. Nihon Ishinkin Gakkai Zasshi. 2006; 47(2):113-7. DOI: 10.3314/jjmm.47.113. View

10.

Lindsley M, Hurst S, Iqbal N, Morrison C . Rapid identification of dimorphic and yeast-like fungal pathogens using specific DNA probes. J Clin Microbiol. 2001; 39(10):3505-11. PMC: 88380. DOI: 10.1128/JCM.39.10.3505-3511.2001. View

11.

Bialek R, Kern J, Herrmann T, Tijerina R, Cecenas L, Reischl U . PCR assays for identification of Coccidioides posadasii based on the nucleotide sequence of the antigen 2/proline-rich antigen. J Clin Microbiol. 2004; 42(2):778-83. PMC: 344486. DOI: 10.1128/JCM.42.2.778-783.2004. View