Characterization of the Major Integral Protein of Vacuolar Membrane

Overview

Journal Plant Physiol

Specialty Physiology

Date 1992 Apr 1

PMID 16668783

Citations 34

Authors

M Maeshima

Affiliations

Soon will be listed here.

Abstract

The vacuolar membrane of radish (Raphanus sativus) taproot contained a large quantity of a protein of 23 kilodaltons that accounted for more than 25% of the total membrane proteins. The protein, tentatively named VM 23, was purified and characterized. VM 23 tends to aggregate at high temperature even in the presence of 1% sodium dodecyl sulfate. The apparent molecular size of VM 23 was estimated to be about 400 kilodaltons by polyacrylamide gel electrophoresis in the presence of 0.1% Triton X-100. VM 23 was partially extracted from the vacuolar membranes with chloroform:methanol, indicating its high hydrophobicity. The hydrophobic carboxyl modifier N,N'-dicyclohexylcarbodiimide bound covalently to VM 23. The results suggest that VM 23 may act as a secondary transport system coupled with the proton transport. The antibody against radish VM 23 reacted with the major proteins in the vacuolar membranes of mung bean (Vigna radiata) and castor bean (Ricinus communis) hypocotyls and pumpkin (Cucurbita moschata) epicotyl, but not with that of sugar beet (Beta vulgaris) taproot. VM 23 comigrated with vacuolar H(+)-pyrophosphatase on sucrose density gradient centrifugation after sonication of membranes, indicating that it is associated with the vacuolar membrane.

Citing Articles

A Review of Plant Vacuoles: Formation, Located Proteins, and Functions.

Tan X, Li K, Wang Z, Zhu K, Tan X, Cao J Plants (Basel). 2019; 8(9).

PMID: 31491897 PMC: 6783984. DOI: 10.3390/plants8090327.

Vacuolar Transporters - Companions on a Longtime Journey.

Martinoia E Plant Physiol. 2018; 176(2):1384-1407.

PMID: 29295940 PMC: 5813537. DOI: 10.1104/pp.17.01481.

Accumulation of TIP2;2 Aquaporin during Dark Adaptation Is Partially PhyA Dependent in Roots of Arabidopsis Seedlings.

Uenishi Y, Nakabayashi Y, Tsuchihira A, Takusagawa M, Hashimoto K, Maeshima M Plants (Basel). 2016; 3(1):177-95.

PMID: 27135499 PMC: 4844315. DOI: 10.3390/plants3010177.

Plasticity between MyoC- and MyoA-glideosomes: an example of functional compensation in Toxoplasma gondii invasion.

Frenal K, Marq J, Jacot D, Polonais V, Soldati-Favre D PLoS Pathog. 2014; 10(10):e1004504.

PMID: 25393004 PMC: 4231161. DOI: 10.1371/journal.ppat.1004504.

Arabidopsis PCR2 is a zinc exporter involved in both zinc extrusion and long-distance zinc transport.

Song W, Choi K, Kim D, Geisler M, Park J, Vincenzetti V Plant Cell. 2010; 22(7):2237-52.

PMID: 20647347 PMC: 2929092. DOI: 10.1105/tpc.109.070185.

References

Matsuura-Endo C, Maeshima M, Yoshida S . Subunit composition of vacuolar membrane H(+)-ATPase from mung bean. Eur J Biochem. 1990; 187(3):745-51. DOI: 10.1111/j.1432-1033.1990.tb15362.x. View

Maeshima M, Beevers H . Purification and properties of glyoxysomal lipase from castor bean. Plant Physiol. 1985; 79(2):489-93. PMC: 1074912. DOI: 10.1104/pp.79.2.489. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Maeshima M, Yoshida S . Purification and properties of vacuolar membrane proton-translocating inorganic pyrophosphatase from mung bean. J Biol Chem. 1989; 264(33):20068-73. View

Zimniak L, Dittrich P, Gogarten J, Kibak H, Taiz L . The cDNA sequence of the 69-kDa subunit of the carrot vacuolar H+-ATPase. Homology to the beta-chain of F0F1-ATPases. J Biol Chem. 1988; 263(19):9102-12. View

Parry R, Turner J, Rea P . High purity preparations of higher plant vacuolar H+-ATPase reveal additional subunits. Revised subunit composition. J Biol Chem. 1989; 264(33):20025-32. View

Rea P, Griffith C, Sanders D . Purification of the N,N'-dicyclohexylcarbodiimide-binding proteolipid of a higher plant tonoplast H+-ATPase. J Biol Chem. 1987; 262(30):14745-52. View

Hjelmeland L . The design and synthesis of detergents for membrane biochemistry. Methods Enzymol. 1986; 124:135-64. DOI: 10.1016/0076-6879(86)24013-6. View

Manolson M, Ouellette B, Filion M, Poole R . cDNA sequence and homologies of the "57-kDa" nucleotide-binding subunit of the vacuolar ATPase from Arabidopsis. J Biol Chem. 1988; 263(34):17987-94. View

10.

Tomida M, Kondo Y, MORIYAMA R, Machida H, Makino S . Effect of stilbenedisulfonate binding on the state of association of the membrane-spanning domain of band 3 from bovine erythrocyte membrane. Biochim Biophys Acta. 1988; 943(3):493-500. DOI: 10.1016/0005-2736(88)90382-3. View

11.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

12.

Sarafian V, Poole R . Purification of an h-translocating inorganic pyrophosphatase from vacuole membranes of red beet. Plant Physiol. 1989; 91(1):34-8. PMC: 1061948. DOI: 10.1104/pp.91.1.34. View

13.

Johnson K, Herman E, Chrispeels M . An abundant, highly conserved tonoplast protein in seeds. Plant Physiol. 1989; 91(3):1006-13. PMC: 1062109. DOI: 10.1104/pp.91.3.1006. View

14.

Uchida E, Ohsumi Y, Anraku Y . Purification and properties of H+-translocating, Mg2+-adenosine triphosphatase from vacuolar membranes of Saccharomyces cerevisiae. J Biol Chem. 1985; 260(2):1090-5. View

15.

Maeshima M . Oligomeric structure of H(+)-translocating inorganic pyrophosphatase of plant vacuoles. Biochem Biophys Res Commun. 1990; 168(3):1157-62. DOI: 10.1016/0006-291x(90)91150-q. View

16.

Johnson K, Hofte H, Chrispeels M . An intrinsic tonoplast protein of protein storage vacuoles in seeds is structurally related to a bacterial solute transporter (GIpF). Plant Cell. 1990; 2(6):525-32. PMC: 159908. DOI: 10.1105/tpc.2.6.525. View

17.

Maeshima M . H(+)-translocating inorganic pyrophosphatase of plant vacuoles. Inhibition by Ca2+, stabilization by Mg2+ and immunological comparison with other inorganic pyrophosphatases. Eur J Biochem. 1991; 196(1):11-7. DOI: 10.1111/j.1432-1033.1991.tb15779.x. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View