Characteristics of a Membrane-associated Lipoxygenase in Tomato Fruit

Overview

Journal Plant Physiol

Specialty Physiology

Date 1990 Nov 1

PMID 16667821

Citations 24

Authors

J F Todd

G Paliyath

J E Thompson

Affiliations

Soon will be listed here.

Abstract

Microsomal membranes isolated from the pericarp of maturegreen tomato (Lycopersicon esculentum) fruit rapidly metabolize exogenous radiolabeled linoleic acid into fatty acid oxidation products at 22 degrees C. The reaction is strongly inhibited by n-propyl gallate, an inhibitor of lipoxygenase. The membranes also rapidly metabolize 16:0/18:2(*) phosphatidylcholine into radiolabeled oxidation products that comigrate on TLC plates with those formed from free linoleic acid. At 30 degrees C, the formation of fatty acid oxidation products from 16:0/18:2(*) phosphatidylcholine is slower, and there is an initial accumulation of radiolabeled linoleic acid that is not evident at 22 degrees C, which can be attributed to the action of lipolytic acyl hydrolase. Radiolabeled phosphatidic acid and diacylglycerol are also formed during metabolism of 16:0/18:2(*) phosphatidylcholine by the microsomal membranes, and there is no breakdown of either linoleic acid or phosphatidylcholine by heat-denatured membranes. When Triton X-100 treated membranes were used, the same patterns of metabolite formation from radiolabeled linoleic acid and 16:0/18:2(*) phosphatidylcholine were observed. Thus, the enzymes mediating the breakdown of these radiolabeled compounds appear to be tightly associated with the membranes. Collectively, the data indicate that there is a lipoxygenase associated with microsomal membranes from tomato fruit that utilizes free fatty acid substrate released from phospholipids. The microsomal lipoxygenase is strongly active over a pH range of 4.5 to 8.0, comprises approximately 38% of the total (microsomal plus soluble) lipoxygenase activity in the tissue, has an apparent K(m) of 0.52 millimolar and an apparent V(max) of 0.186 millimoles per minute per milligram of protein. The membranous enzyme also cross-reacts with polyclonal antibodies raised against soybean lipoxygenase-1 and has an apparent molecular mass of 100 kilodaltons.

Citing Articles

Alleviation of Cadmium and Nickel Toxicity and Phyto-Stimulation of Tomato Plant L. by Endophytic and .

Badawy I, Hmed A, Sofy M, Al-Mokadem A Plants (Basel). 2022; 11(15).

PMID: 35956496 PMC: 9370581. DOI: 10.3390/plants11152018.

The Efficacies of 1-Methylcyclopropene and Chitosan Nanoparticles in Preserving the Postharvest Quality of Damask Rose and Their Underlying Biochemical and Physiological Mechanisms.

Ali E, Issa A, Al-Yasi H, Hessini K, Hassan F Biology (Basel). 2022; 11(2).

PMID: 35205108 PMC: 8869683. DOI: 10.3390/biology11020242.

Ameliorating the Adverse Effects of Infecting Tomato Plants in Egypt by Boosting Immunity in Tomato Plants Using Zinc Oxide Nanoparticles.

Sofy A, Sofy M, Hmed A, Dawoud R, Alnaggar A, Soliman A Molecules. 2021; 26(5).

PMID: 33801530 PMC: 7958966. DOI: 10.3390/molecules26051337.

Transcriptome analysis reveals mechanism of early ripening in Kyoho grape with hydrogen peroxide treatment.

Guo D, Wang Z, Pei M, Guo L, Yu Y BMC Genomics. 2020; 21(1):784.

PMID: 33176674 PMC: 7657363. DOI: 10.1186/s12864-020-07180-y.

Improving Regulation of Enzymatic and Non-Enzymatic Antioxidants and Stress-Related Gene Stimulation in -Infected Cucumber Plants Treated with Glycine Betaine, Chitosan and Combination.

Sofy A, Dawoud R, Sofy M, Mohamed H, Hmed A, El-Dougdoug N Molecules. 2020; 25(10).

PMID: 32429524 PMC: 7288169. DOI: 10.3390/molecules25102341.

References

Reddanna P, Whelan J, Reddy P, Reddy C . Isolation and characterization of 5-lipoxygenase from tulip bulbs. Biochem Biophys Res Commun. 1988; 157(3):1348-51. DOI: 10.1016/s0006-291x(88)81023-4. View

Siedow J, Girvin M . Alternative Respiratory Pathway: ITS ROLE IN SEED RESPIRATION AND ITS INHIBITION BY PROPYL GALLATE. Plant Physiol. 1980; 65(4):669-74. PMC: 440403. DOI: 10.1104/pp.65.4.669. View

Funk M, Whitney M, Hausknecht E, OBrien E . Resolution of the isoenzymes of soybean lipoxygenase using isoelectric focusing and chromatofocusing. Anal Biochem. 1985; 146(1):246-51. DOI: 10.1016/0003-2697(85)90422-1. View

Mack A, Peterman T, Siedow J . Lipoxygenase isozymes in higher plants: biochemical properties and physiological role. Isozymes Curr Top Biol Med Res. 1987; 13:127-54. View

Leibowitz M, Johnson M . Relation of lipid peroxidation to loss of cations trapped in liposomes. J Lipid Res. 1971; 12(6):662-70. View

Peterman T, Siedow J . Behavior of Lipoxygenase during Establishment, Senescence, and Rejuvenation of Soybean Cotyledons. Plant Physiol. 1985; 78(4):690-5. PMC: 1064805. DOI: 10.1104/pp.78.4.690. View

Bligny R, Foray M, Roby C, Douce R . Transport and phosphorylation of choline in higher plant cells. Phosphorus-31 nuclear magnetic resonance studies. J Biol Chem. 1989; 264(9):4888-95. View

Bligny R, Douce R . Calcium-dependent lipolytic acyl-hydrolase activity in purified plant mitochondria. Biochim Biophys Acta. 1978; 529(3):419-28. DOI: 10.1016/0005-2760(78)90086-3. View

Matsushita S . Specific interactions of linoleic acid hydroperoxides and their secondary degraded products with enzyme proteins. J Agric Food Chem. 1975; 23(2):150-4. DOI: 10.1021/jf60198a049. View

10.

Schewe T, RAPOPORT S, Kuhn H . Enzymology and physiology of reticulocyte lipoxygenase: comparison with other lipoxygenases. Adv Enzymol Relat Areas Mol Biol. 1986; 58:191-272. DOI: 10.1002/9780470123041.ch6. View

11.

Paliyath G, Thompson J . Senescence-Related Changes in ATP-Dependent Uptake of Calcium into Microsomal Vesicles from Carnation Petals. Plant Physiol. 1988; 88(2):295-302. PMC: 1055571. DOI: 10.1104/pp.88.2.295. View

12.

Fobel M, Lynch D, Thompson J . Membrane deterioration in senescing carnation flowers : coordinated effects of phospholipid degradation and the action of membranous lipoxygenase. Plant Physiol. 1987; 85(1):204-11. PMC: 1054230. DOI: 10.1104/pp.85.1.204. View

13.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

14.

Peterman T, Siedow J . Immunological comparison of lipoxygenase isozymes-1 and -2 with soybean seedling lipoxygenases. Arch Biochem Biophys. 1985; 238(2):476-83. DOI: 10.1016/0003-9861(85)90190-0. View

15.

Kanofsky J, AXELROD B . Singlet oxygen production by soybean lipoxygenase isozymes. J Biol Chem. 1986; 261(3):1099-104. View

16.

Martin T . Formation of diacylglycerol by a phospholipase D-phosphatidate phosphatase pathway specific for phosphatidylcholine in endothelial cells. Biochim Biophys Acta. 1988; 962(3):282-96. DOI: 10.1016/0005-2760(88)90258-5. View

17.

Jensenius J, Andersen I, Hau J, Crone M, Koch C . Eggs: conveniently packaged antibodies. Methods for purification of yolk IgG. J Immunol Methods. 1981; 46(1):63-8. DOI: 10.1016/0022-1759(81)90333-1. View

18.

Paliyath G, Thompson J . Calcium- and calmodulin-regulated breakdown of phospholipid by microsomal membranes from bean cotyledons. Plant Physiol. 1987; 83(1):63-8. PMC: 1056300. DOI: 10.1104/pp.83.1.63. View

19.

Vernooy-Gerritsen M, Leunissen J, Veldink G, Vliegenthart J . Intracellular localization of lipoxygenases-1 and -2 in germinating soybean seeds by indirect labeling with protein a-colloidal gold complexes. Plant Physiol. 1984; 76(4):1070-8. PMC: 1064436. DOI: 10.1104/pp.76.4.1070. View