Isozymes of Superoxide Dismutase in Mitochondria and Peroxisomes Isolated from Petals of Carnation (Dianthus Caryophyllus) During Senescence

Overview

Journal Plant Physiol

Specialty Physiology

Date 1990 Nov 1

PMID 16667815

Citations 24

Authors

M J Droillard

A Paulin

Affiliations

Soon will be listed here.

Abstract

The balance between reactions involving free radicals and processes which ameliorate their effect plays an important role in the regulation of plant senescence. In this study a method was developed to isolate peroxisomes and mitochondria from carnation (Dianthus caryophyllus L. cv Ember) petals. Based on electron microscopy and marker enzyme levels, the proportion of peroxisomes to mitochondria increases during senescence. The superoxide dismutase (SOD) content of these fractions was examined. Mitochondria and peroxisomes were shown to contain two electrophoretically distinct SODs, a manganese-, and an ironcontaining SOD. The Mn- and Fe-SOD were found to have relative molecular weights of 75,000 and 48,000 and isoelectric points of 4.85 and 5.00, respectively. The presence of a Fe-SOD in mitochondria and peroxisomes is unique because this enzyme is usually located in chloroplasts. The activity of these two isoenzymes decreased during senescence in mitochondria but remained high in peroxisomes from senescent tissue. It is suggested that peroxisomes play a particular role in the process of senescence.

Citing Articles

Functional characterization of a manganese superoxide dismutase from Avicennia marina: insights into its role in salt, hydrogen peroxide, and heavy metal tolerance.

Abedi H, Shahpiri A Sci Rep. 2024; 14(1):406.

PMID: 38172216 PMC: 10764323. DOI: 10.1038/s41598-023-50851-5.

Interplay among Antioxidant System, Hormone Profile and Carbohydrate Metabolism during Bud Dormancy Breaking in a High-Chill Peach Variety.

Hernandez J, Diaz-Vivancos P, Acosta-Motos J, Alburquerque N, Martinez D, Carrera E Antioxidants (Basel). 2021; 10(4).

PMID: 33916531 PMC: 8066612. DOI: 10.3390/antiox10040560.

Plant Peroxisomes: A Factory of Reactive Species.

Corpas F, Gonzalez-Gordo S, Palma J Front Plant Sci. 2020; 11:853.

PMID: 32719691 PMC: 7348659. DOI: 10.3389/fpls.2020.00853.

Identification of novel superoxide dismutase isoenzymes in the olive (Olea europaea L.) pollen.

Zafra A, Castro A, de Dios Alche J BMC Plant Biol. 2018; 18(1):114.

PMID: 29884131 PMC: 5994013. DOI: 10.1186/s12870-018-1328-z.

Plant peroxisomes: A nitro-oxidative cocktail.

Corpas F, Barroso J, Palma J, Rodriguez-Ruiz M Redox Biol. 2017; 11:535-542.

PMID: 28092771 PMC: 5238456. DOI: 10.1016/j.redox.2016.12.033.

References

Bridges S, Salin M . Distribution of iron-containing superoxide dismutase in vascular plants. Plant Physiol. 1981; 68(2):275-8. PMC: 427475. DOI: 10.1104/pp.68.2.275. View

Kanematsu S, Asada K . Ferric and manganic superoxide dismutases in Euglena gracilis. Arch Biochem Biophys. 1979; 195(2):535-45. DOI: 10.1016/0003-9861(79)90380-1. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Del Rio L, Fernandez V, Ruperez F, Sandalio L, Palma J . NADH Induces the Generation of Superoxide Radicals in Leaf Peroxisomes. Plant Physiol. 1989; 89(3):728-31. PMC: 1055913. DOI: 10.1104/pp.89.3.728. View

Tolbert N . Isolation of subcellular organelles of metabolism on isopycnic sucrose gradients. Methods Enzymol. 1974; 31:734-46. DOI: 10.1016/0076-6879(74)31077-4. View

Tolbert N, Essner E . Microbodies: peroxisomes and glyoxysomes. J Cell Biol. 1981; 91(3 Pt 2):271s-283s. PMC: 2112809. DOI: 10.1083/jcb.91.3.271s. View

Fridovich I . Biological effects of the superoxide radical. Arch Biochem Biophys. 1986; 247(1):1-11. DOI: 10.1016/0003-9861(86)90526-6. View

Fridovich I . Superoxide dismutases. Annu Rev Biochem. 1975; 44:147-59. DOI: 10.1146/annurev.bi.44.070175.001051. View

Hatch M . A simple spectrophotometric assay for fumarate hydratase in crude tissue extracts. Anal Biochem. 1978; 85(1):271-5. DOI: 10.1016/0003-2697(78)90299-3. View

10.

Kellogg 3rd E, Fridovich I . Superoxide, hydrogen peroxide, and singlet oxygen in lipid peroxidation by a xanthine oxidase system. J Biol Chem. 1975; 250(22):8812-7. View

11.

Schwitzguebel J, Siegenthaler P . Purification of peroxisomes and mitochondria from spinach leaf by percoll gradient centrifugation. Plant Physiol. 1984; 75(3):670-4. PMC: 1066974. DOI: 10.1104/pp.75.3.670. View

12.

Becana M, Paris F, Sandalio L, Del Rio L . Isoenzymes of Superoxide Dismutase in Nodules of Phaseolus vulgaris L., Pisum sativum L., and Vigna unguiculata (L.) Walp. Plant Physiol. 1989; 90(4):1286-92. PMC: 1061884. DOI: 10.1104/pp.90.4.1286. View

13.

Hassan H . Biosynthesis and regulation of superoxide dismutases. Free Radic Biol Med. 1988; 5(5-6):377-85. DOI: 10.1016/0891-5849(88)90111-6. View

14.

Beauchamp C, Fridovich I . Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971; 44(1):276-87. DOI: 10.1016/0003-2697(71)90370-8. View

15.

Nohl H, Jordan W . The mitochondrial site of superoxide formation. Biochem Biophys Res Commun. 1986; 138(2):533-9. DOI: 10.1016/s0006-291x(86)80529-0. View

16.

Salin M, Bridges S . Isolation and Characterization of an Iron-Containing Superoxide Dismutase From Water Lily, Nuphar luteum. Plant Physiol. 1982; 69(1):161-5. PMC: 426167. DOI: 10.1104/pp.69.1.161. View

17.

Halliwell B, Gutteridge J . Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J. 1984; 219(1):1-14. PMC: 1153442. DOI: 10.1042/bj2190001. View

18.

Droillard M, Bureau D, Paulin A, Daussant J . Identification of different classes of superoxide dismutase in carnation petals. Electrophoresis. 1989; 10(1):46-8. DOI: 10.1002/elps.1150100111. View

19.

Sandalio L, Del Rio L . Intraorganellar distribution of superoxide dismutase in plant peroxisomes (glyoxysomes and leaf peroxisomes). Plant Physiol. 1988; 88(4):1215-8. PMC: 1055743. DOI: 10.1104/pp.88.4.1215. View

20.

Halliwell B . Biochemical mechanisms accounting for the toxic action of oxygen on living organisms: the key role of superoxide dismutase. Cell Biol Int Rep. 1978; 2(2):113-28. DOI: 10.1016/0309-1651(78)90032-2. View