Altered Cell Surface Glycoproteins in Phytohemagglutinin-resistant Mutants of Chinese Hamster Ovary Cells

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 1975 May 6

PMID 166678

Citations 8

Authors

R L Juliano

P Stanley

Affiliations

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Abstract

Purified membranes from surface-labelled phytohemagglutinin-resistant (Pha(R) and wild-type chinese hamster ovary cells have been analysed by sodium dodecyl sulphate gel electrophoresis. Gel patterns were compared for cells labelled via galactose oxidase and B-3H4 or lactoperoxidase and radioactive iodide. The results suggest that Pha-R cells are altered in the carbohydrate portion of a number of their membrane glycoproteins.

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