Photoregulation of Beta-Tubulin MRNA Abundance in Etiolated Oat and Barley Seedlings

Overview

Journal Plant Physiol

Specialty Physiology

Date 1990 Jul 1

PMID 16667578

Citations 12

Authors

J T Colbert

S A Costigan

Z Zhao

Affiliations

Soon will be listed here.

Abstract

The effect of light on the abundance of beta-tubulin mRNA was measured in etiolated Avena sativa L. and Hordeum vulgare L. seedlings. Slot blot analysis employing an oat beta-tubulin cDNA clone was used to measure beta-tubulin mRNA levels. White light induced a 45% decrease in oat beta-tubulin mRNA abundance by 2 hours after transfer. A saturating red light pulse induced 40 and 55% decreases in beta-tubulin mRNA levels in oats and barley, respectively. Recovery of beta-tubulin mRNA levels was observed after a red light pulse but not after transfer to continuous white light. The red light induced decrease in oat beta-tubulin mRNA abundance was not reversible by a subsequent far-red light treatment. The mesocotyl portion of etiolated oat seedlings exhibited a more dramatic decrease in beta-tubulin mRNA abundance in response to red light than did the coleoptile portion. The results indicate that the well-documented effects of red light on the growth of etiolated seedlings are accompanied by changes in the expression of the beta-tubulin genes.

Citing Articles

Isolation and Characterization of Three Genes Negatively Regulated by Phytochrome Action in Lemna gibba.

Okubara P, Tobin E Plant Physiol. 1991; 96(4):1237-45.

PMID: 16668325 PMC: 1080921. DOI: 10.1104/pp.96.4.1237.

Lupinus albus gamma-tubulin: mRNA and protein accumulation during development and in response to darkness.

Saibo N, Van Der Straeten D, Rodrigues-Pousada C Planta. 2004; 219(2):201-11.

PMID: 14986143 DOI: 10.1007/s00425-004-1218-5.

Phototropin 1 is required for high-fluence blue-light-mediated mRNA destabilization.

Folta K, Kaufman L Plant Mol Biol. 2003; 51(4):609-18.

PMID: 12650626 DOI: 10.1023/a:1022393406204.

Red Light-Independent Instability of Oat Phytochrome mRNA in Vivo.

Seeley K, Byrne D, Colbert J Plant Cell. 1992; 4(1):29-38.

PMID: 12297628 PMC: 160103. DOI: 10.1105/tpc.4.1.29.

Characterization of protein and transcript levels of the chaperonin containing tailless complex protein-1 and tubulin during light-regulated growth of oat seedlings.

Moser M, Schafer E, Ehmann B Plant Physiol. 2000; 124(1):313-20.

PMID: 10982445 PMC: 59145. DOI: 10.1104/pp.124.1.313.

References

Dean C, Elzen P, Tamaki S, Dunsmuir P, Bedbrook J . Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multi-gene family. EMBO J. 1985; 4(12):3055-61. PMC: 554622. DOI: 10.1002/j.1460-2075.1985.tb04045.x. View

Lissemore J, Quail P . Rapid transcriptional regulation by phytochrome of the genes for phytochrome and chlorophyll a/b-binding protein in Avena sativa. Mol Cell Biol. 1988; 8(11):4840-50. PMC: 365577. DOI: 10.1128/mcb.8.11.4840-4850.1988. View

Kay S, Keith B, Shinozaki K, Chye M, Chua N . The rice phytochrome gene: structure, autoregulated expression, and binding of GT-1 to a conserved site in the 5' upstream region. Plant Cell. 1989; 1(3):351-60. PMC: 159767. DOI: 10.1105/tpc.1.3.351. View

Colbert J, Hershey H, Quail P . Autoregulatory control of translatable phytochrome mRNA levels. Proc Natl Acad Sci U S A. 1983; 80(8):2248-52. PMC: 393796. DOI: 10.1073/pnas.80.8.2248. View

Cleveland D, Lopata M, Sherline P, Kirschner M . Unpolymerized tubulin modulates the level of tubulin mRNAs. Cell. 1981; 25(2):537-46. DOI: 10.1016/0092-8674(81)90072-6. View

Mosinger E, Batschauer A, Apel K, Schafer E, Briggs W . Phytochrome regulation of greening in barley : effects on mRNA abundance and on transcriptional activity of isolated nuclei. Plant Physiol. 1988; 86(3):706-10. PMC: 1054556. DOI: 10.1104/pp.86.3.706. View

Schedl T, Burland T, Gull K, Dove W . Cell cycle regulation of tubulin RNA level, tubulin protein synthesis, and assembly of microtubules in Physarum. J Cell Biol. 1984; 99(1 Pt 1):155-65. PMC: 2275639. DOI: 10.1083/jcb.99.1.155. View

Smith H, Jackson G . Rapid phytochrome regulation of wheat seedling extension: light pretreatment extends coupling time, increases response lag, and decreases light sensitivity. Plant Physiol. 1987; 84(4):1059-62. PMC: 1056727. DOI: 10.1104/pp.84.4.1059. View

Bustos M, Guiltinan M, Cyr R, Ahdoot D, Fosket D . Light Regulation of beta-Tubulin Gene Expression during Internode Development in Soybean (Glycine max [L.] Merr.). Plant Physiol. 1989; 91(3):1157-61. PMC: 1062134. DOI: 10.1104/pp.91.3.1157. View

10.

Gay D, Yen T, Lau J, Cleveland D . Sequences that confer beta-tubulin autoregulation through modulated mRNA stability reside within exon 1 of a beta-tubulin mRNA. Cell. 1987; 50(5):671-9. DOI: 10.1016/0092-8674(87)90325-4. View

11.

Mandoli D, Briggs W . Phytochrome control of two low-irradiance responses in etiolated oat seedlings. Plant Physiol. 1981; 67(4):733-9. PMC: 425763. DOI: 10.1104/pp.67.4.733. View

12.

Yen T, Machlin P, Cleveland D . Autoregulated instability of beta-tubulin mRNAs by recognition of the nascent amino terminus of beta-tubulin. Nature. 1988; 334(6183):580-5. DOI: 10.1038/334580a0. View

13.

Schaer J, Mandoli D, Briggs W . Phytochrome-mediated cellular photomorphogenesis. Plant Physiol. 1983; 72(3):706-12. PMC: 1066306. DOI: 10.1104/pp.72.3.706. View

14.

Tsai F, Coruzzi G . Dark-induced and organ-specific expression of two asparagine synthetase genes in Pisum sativum. EMBO J. 1990; 9(2):323-32. PMC: 551669. DOI: 10.1002/j.1460-2075.1990.tb08114.x. View

15.

Schloss J, Silflow C, Rosenbaum J . mRNA abundance changes during flagellar regeneration in Chlamydomonas reinhardtii. Mol Cell Biol. 1984; 4(3):424-34. PMC: 368719. DOI: 10.1128/mcb.4.3.424-434.1984. View