Phosphoinositides in Barley Aleurone Layers and Gibberellic Acid-induced Changes in Metabolism

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 Dec 1

PMID 16667174

Citations 10

Authors

P P Murthy

J M Renders

L M Keranen

Affiliations

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Abstract

Phospholipids of barley (Hordeum vulgare L. cv Himalaya) aleurone layers were labeled with myo-[2-(3)H]inositol or [(32)Pi], extracted, and analyzed by physical (chromatography) and chemical (deacylation) techniques. Three phospholipids were found to incorporate both myo-[2-(3)H]inositol and [(32)Pi]-phosphatidylinositol, phosphatidylinositol-monophosphate, and phosphatidylinositol-bisphosphate. Stimulation of [(3)H]inositol prelabeled aleurone layers with GA(3) showed enhanced incorporation of label into phosphatidylinositol within 30 seconds and subsequent rapid breakdown. Stimulation of phosphatidylinositol labeling observed in these studies is the earliest response of aleurone cells to gibberellic acid reported.

Citing Articles

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Evidence of two isomers of phosphatidylinositol in plant tissue.

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