Interruption of Somatic Embryogenesis in Daucus Carota L. by 5-Bromodeoxyuridine

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 Jul 1

PMID 16666898

Citations 2

Authors

J C Thomas

C Nessler

F Katterman

Affiliations

Soon will be listed here.

Abstract

Embryogenic Daucus carota L. cells grown in 9 micromolar 2.4-dichlorophenoxyacetic acid are resistant to greater than 5 micromolar 5-bromodeoxyuridine (BrdU). In contrast, 5 micromolar BrdU strongly inhibits somatic embryogenesis within 24 hours after transfer of cells to an auxin-free medium. DNA synthesis rates in control and BrdU-treated cultures are rapid and similar; however, the DNA content does not reach levels as great in the presence of BrdU as in control cultures. BrdU substitutes for thymidine in the DNA in 28% of the available sites 48 hours after auxin removal. Following DNA repair, somatic embryogenesis resumes. BrdU DNA incorporation leads to somatic embryogenesis inhibition and provides an alternative to auxin treatment for the interruption of carrot cell culture differentiation.

Citing Articles

Carrot (Daucus carota) hypocotyl transformation usingAgrobacterium tumefaciens.

Thomas J, Guiltinan M, Bustos S, Thomas T, Nessler C Plant Cell Rep. 2013; 8(6):354-7.

PMID: 24233274 DOI: 10.1007/BF00716672.

Influence of NaCl on Growth, Proline, and Phosphoenolpyruvate Carboxylase Levels in Mesembryanthemum crystallinum Suspension Cultures.

Thomas J, De Armond R, Bohnert H Plant Physiol. 1992; 98(2):626-31.

PMID: 16668687 PMC: 1080236. DOI: 10.1104/pp.98.2.626.

References

Sung Z, Okimoto R . Coordinate gene expression during somatic embryogenesis in carrots. Proc Natl Acad Sci U S A. 1983; 80(9):2661-5. PMC: 393887. DOI: 10.1073/pnas.80.9.2661. View

Choi J, Liu L, Borkird C, Sung Z . Cloning of genes developmentally regulated during plant embryogenesis. Proc Natl Acad Sci U S A. 1987; 84(7):1906-10. PMC: 304550. DOI: 10.1073/pnas.84.7.1906. View

Kornberg A . DNA replication. J Biol Chem. 1988; 263(1):1-4. View

Gorman C, Moffat L, Howard B . Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982; 2(9):1044-51. PMC: 369897. DOI: 10.1128/mcb.2.9.1044-1051.1982. View

Hernandez P, Lamm S, Bjerknes C, Hof J . Replication termini in the rDNA of synchronized pea root cells (Pisum sativum). EMBO J. 1988; 7(2):303-8. PMC: 454311. DOI: 10.1002/j.1460-2075.1988.tb02813.x. View

Rutter W, Pictet R, MORRIS P . Toward molecular mechanisms of developmental processes. Annu Rev Biochem. 1973; 42:601-46. DOI: 10.1146/annurev.bi.42.070173.003125. View

Luk D, Bick M . Determination of 5'-bromodeoxyuridine in DNA by buoyant density. Anal Biochem. 1977; 77(2):346-9. DOI: 10.1016/0003-2697(77)90247-0. View

SZYSZKO J, Pietrzykowska I, Twardowski T, SHUGAR D . Identification of uracil as a major lesion in E. coli DNA following the incorporation of 5-bromouracil, and some of the accompanying effects. Mutat Res. 1983; 108(1-3):13-27. DOI: 10.1016/0027-5107(83)90106-9. View

Hutchison C, Cox R, Drepaul R, Gomperts M, Ford C . Periodic DNA synthesis in cell-free extracts of Xenopus eggs. EMBO J. 1987; 6(7):2003-10. PMC: 553589. DOI: 10.1002/j.1460-2075.1987.tb02464.x. View

10.

Gamborg O, Miller R, Ojima K . Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968; 50(1):151-8. DOI: 10.1016/0014-4827(68)90403-5. View

11.

Sung Z, Okimoto R . Embryonic proteins in somatic embryos of carrot. Proc Natl Acad Sci U S A. 1981; 78(6):3683-7. PMC: 319635. DOI: 10.1073/pnas.78.6.3683. View

12.

Flickinger R, Grabar F, Kundahl E, SHEPHERD G . Replicon size increase due to 5-bromodeoxyuridine incorporation. Cell Biol Int Rep. 1980; 4(2):129-35. DOI: 10.1016/0309-1651(80)90067-3. View

13.

Fromm M, Taylor L, Walbot V . Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc Natl Acad Sci U S A. 1985; 82(17):5824-8. PMC: 390645. DOI: 10.1073/pnas.82.17.5824. View

14.

Halperin W, Jensen W . Ultrastructural changes during growth and embryogenesis in carrot cell cultures. J Ultrastruct Res. 1967; 18(3):428-43. DOI: 10.1016/s0022-5320(67)80128-x. View

15.

Biswas D, Lyons J, TASHJIAN Jr A . Induction of prolactin synthesis in rat pituitary tumor cells by 5-bromodeoxyuridine. Cell. 1977; 11(2):431-9. DOI: 10.1016/0092-8674(77)90061-7. View

16.

Vytasek R . A sensitive fluorometric assay for the determination of DNA. Anal Biochem. 1982; 120(2):243-8. DOI: 10.1016/0003-2697(82)90342-6. View

17.

Frearson E, Power J, Cocking E . The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol. 1973; 33(1):130-7. DOI: 10.1016/0012-1606(73)90169-3. View