Quantitation of Indoleacetic Acid Conjugates in Bean Seeds by Direct Tissue Hydrolysis

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 Jun 1

PMID 16666783

Citations 15

Authors

K Bialek

J D Cohen

Affiliations

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Abstract

Gas chromatography-selected ion monitoring-mass spectral analysis using [(13)C(6)]indole-3-acetic acid (IAA) as an internal standard provides an effective means for quantitation of IAA liberated during direct strong basic hydrolysis of bean (Phaseolus vulgaris L.) seed powder, provided that extra precautions are undertaken to exclude oxygen from the reaction vial. Direct seed powder hydrolysis revealed that the major portion of amide IAA conjugates in bean seeds are not extractable by aqueous acetone, the solvent used commonly for IAA conjugate extraction from seeds and other plant tissues. Strong basic hydrolysis of plant tissue can be used to provide new information on IAA content.

Citing Articles

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Interaction of plant growth regulators and reactive oxygen species to regulate petal senescence in wallflowers (Erysimum linifolium).

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Quantitative evaluation of IAA conjugate pools in Arabidopsis thaliana.

Yu P, Lor P, Ludwig-Muller J, Hegeman A, Cohen J Planta. 2014; 241(2):539-48.

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Transport of indole-3-butyric acid and indole-3-acetic acid in Arabidopsis hypocotyls using stable isotope labeling.

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References

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