Hydrolytic Enzyme Activities and Protein Pattern of Avocado Fruit Ripened in Air and in Low Oxygen, with and Without Ethylene

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 May 1

PMID 16666746

Citations 9

Authors

A K Kanellis

T Solomos

A K Mattoo

Affiliations

Soon will be listed here.

Abstract

The effect of 2.5% O(2) atmosphere with and without ethylene on the activities of hydrolytic enzymes associated with cell walls, and total protein profile during ripening of avocado fruits (Persea americana Mill., cv Hass) were investigated. The low 2.5% O(2) atmosphere prevented the rise in the activities of cellulase, polygalacturonase, and acid phosphatase in avocado fruits whose ripening was initiated with ethylene. Addition of 100 microliters per liter ethylene to low O(2) atmosphere did not alter these suppressive effects of 2.5% O(2). Furthermore, 2.5% O(2) atmosphere delayed the development of a number of polypeptides that appear during ripening of avocado fruits while at the same time new polypeptides accumulated. The composition of the extraction buffer and its pH greatly affected the recovery of cellulase activity and its total immunoreactive protein.

Citing Articles

Role of internal atmosphere on fruit ripening and storability-a review.

Paul V, Pandey R J Food Sci Technol. 2014; 51(7):1223-50.

PMID: 24966416 PMC: 4062679. DOI: 10.1007/s13197-011-0583-x.

De novo sequencing, assembly, and analysis of the root transcriptome of Persea americana (Mill.) in response to Phytophthora cinnamomi and flooding.

Reeksting B, Coetzer N, Mahomed W, Engelbrecht J, van den Berg N PLoS One. 2014; 9(2):e86399.

PMID: 24563685 PMC: 3919710. DOI: 10.1371/journal.pone.0086399.

The fading distinctions between classical patterns of ripening in climacteric and non-climacteric fruit and the ubiquity of ethylene-An overview.

Paul V, Pandey R, Srivastava G J Food Sci Technol. 2013; 49(1):1-21.

PMID: 23572821 PMC: 3550874. DOI: 10.1007/s13197-011-0293-4.

Ripening of tomato (Solanum lycopersicum L.). Part II: Regulation by its stem scar region.

Paul V, Pandey R, Srivastava G J Food Sci Technol. 2013; 47(5):527-33.

PMID: 23572681 PMC: 3551105. DOI: 10.1007/s13197-010-0089-y.

McGarvey D, Sirevag R, Christoffersen R Plant Physiol. 1992; 98(2):554-9.

PMID: 16668676 PMC: 1080225. DOI: 10.1104/pp.98.2.554.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Tucker M, Laties G . Interrelationship of Gene Expression, Polysome Prevalence, and Respiration during Ripening of Ethylene and/or Cyanide-Treated Avocado Fruit. Plant Physiol. 1984; 74(2):307-15. PMC: 1066674. DOI: 10.1104/pp.74.2.307. View

Awad M, Young R . Postharvest Variation in Cellulase, Polygalacturonase, and Pectinmethylesterase in Avocado (Persea americana Mill, cv. Fuerte) Fruits in Relation to Respiration and Ethylene Production. Plant Physiol. 1979; 64(2):306-8. PMC: 543076. DOI: 10.1104/pp.64.2.306. View

Pesis E, Fuchs Y, Zauberman G . Cellulase activity and fruit softening in avocado. Plant Physiol. 1978; 61(3):416-9. PMC: 1091880. DOI: 10.1104/pp.61.3.416. View

Gallagher S, Leonard R . Electrophoretic characterization of a detergent-treated plasma membrane fraction from corn roots. Plant Physiol. 1987; 83(2):265-71. PMC: 1056346. DOI: 10.1104/pp.83.2.265. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Alpi A, Beevers H . Proteinases and enzyme stability in crude extracts of castor bean endosperm. Plant Physiol. 1981; 67(3):499-502. PMC: 425713. DOI: 10.1104/pp.67.3.499. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Solomos T, Laties G . Similarities between the Actions of Ethylene and Cyanide in Initiating the Climacteric and Ripening of Avocados. Plant Physiol. 1974; 54(4):506-11. PMC: 367443. DOI: 10.1104/pp.54.4.506. View

10.

Lin C, Key J . Dissocation and reassembly of polyribosomes in relation to protein synthesis in the soybean root. J Mol Biol. 1967; 26(2):237-47. DOI: 10.1016/0022-2836(67)90294-x. View

11.

Burg S, Burg E . Molecular requirements for the biological activity of ethylene. Plant Physiol. 1967; 42(1):144-52. PMC: 1086501. DOI: 10.1104/pp.42.1.144. View

12.

Sacher J . Acid Phosphatase Development during Ripening of Avocado. Plant Physiol. 1975; 55(2):382-5. PMC: 541620. DOI: 10.1104/pp.55.2.382. View

13.

Wray W, Boulikas T, Wray V, Hancock R . Silver staining of proteins in polyacrylamide gels. Anal Biochem. 1981; 118(1):197-203. DOI: 10.1016/0003-2697(81)90179-2. View

14.

Mattoo A, Pick U, Hoffman-Falk H, Edelman M . The rapidly metabolized 32,000-dalton polypeptide of the chloroplast is the "proteinaceous shield" regulating photosystem II electron transport and mediating diuron herbicide sensitivity. Proc Natl Acad Sci U S A. 1981; 78(3):1572-6. PMC: 319173. DOI: 10.1073/pnas.78.3.1572. View

15.

Christoffersen R, Bennett A . Biotinylated proteins as molecular weight standards on Western blots. Anal Biochem. 1986; 152(2):329-32. DOI: 10.1016/0003-2697(86)90417-3. View

16.

ALMIN K, Eriksson K, Jansson C . Enzymic degradation of polymers. II. Viscometric determination of cellulase activity in absolute terms. Biochim Biophys Acta. 1967; 139(2):248-53. DOI: 10.1016/0005-2744(67)90029-0. View

17.

Sachs M, Freeling M, Okimoto R . The anaerobic proteins of maize. Cell. 1980; 20(3):761-7. DOI: 10.1016/0092-8674(80)90322-0. View