Purification and Characterization of Calmodulins from Papaver Somniferum and Euphorbia Lathyris

Overview

Journal Plant Physiol

Specialty Physiology

Date 1989 Feb 1

PMID 16666573

Citations 1

Authors

M P Thompson

G J Piazza

D P Brower

H M Farrell

Affiliations

Soon will be listed here.

Abstract

Calmodulins (CaM) were isolated and characterized from two well-known latex producing plants, Papaver somniferum and Euphorbia lathyris. The molecular weights of both were determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 17,000 comparable to that of bovine brain CaM. Amino acid compositions also compared similarly with those of known CaMs, with regard to the presence of trimethyllysine and the ratio of phenylalanine to tyrosine. The Cornish-Bowden equation (SDeltan) revealed strong statistical correlations of P. somniferum and E. lathyris CaM with those of other plants and animals, although their amino acid compositions were not identical. Both plant CaM stimulated CaM dependent cAMP phosphodiesterase: for Papaver somniferum the K(a) was found to be 1.09 nanomolar and for Euphorbia lathyris, 2.01 nanomolar.

Citing Articles

Where does N(ε)-trimethyllysine for the carnitine biosynthesis in mammals come from?.

Servillo L, Giovane A, Cautela D, Castaldo D, Balestrieri M PLoS One. 2014; 9(1):e84589.

PMID: 24454731 PMC: 3890275. DOI: 10.1371/journal.pone.0084589.

References

Schleicher M, Lukas T, Watterson D . Further Characterization of Calmodulin from the Monocotyledon Barley (Hordeum vulgare). Plant Physiol. 1983; 73(3):666-70. PMC: 1066527. DOI: 10.1104/pp.73.3.666. View

Toda H, Yazawa M, Sakiyama F, Yagi K . Amino acid sequence of calmodulin from wheat germ. J Biochem. 1985; 98(3):833-42. DOI: 10.1093/oxfordjournals.jbchem.a135342. View

Piazza G, Saggese E, Spletzer K . Triterpene Biosynthesis in the Latex of Euphorbia lathyris: Effect of Calmodulin Antagonists and Chlorinated Phenoxy Compounds. Plant Physiol. 1987; 83(1):177-80. PMC: 1056319. DOI: 10.1104/pp.83.1.177. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

Butcher R . Cyclic 3',5'-nucleotide phosphodiesterase from bovine heart. Methods Enzymol. 1974; 38:218-23. DOI: 10.1016/0076-6879(74)38035-4. View

Nessler C, Allen R, Galewsky S . Identification and characterization of latex-specific proteins in opium poppy. Plant Physiol. 1985; 79(2):499-504. PMC: 1074914. DOI: 10.1104/pp.79.2.499. View

Watterson D, Iverson D, Van Eldik L . Spinach calmodulin: isolation, characterization, and comparison with vertebrate calmodulins. Biochemistry. 1980; 19(25):5762-8. DOI: 10.1021/bi00566a015. View

Thompson M, Piazza G, Brower D, BINGHAM E, Farrell Jr H . Isolation and characterization of bovine mammary calmodulin. J Dairy Sci. 1987; 70(8):1551-6. DOI: 10.3168/jds.S0022-0302(87)80182-0. View

Lukas T, Iverson D, Schleicher M, Watterson D . Structural characterization of a higher plant calmodulin : spinacia oleracea. Plant Physiol. 1984; 75(3):788-95. PMC: 1066994. DOI: 10.1104/pp.75.3.788. View

10.

Schreiber W, Sasagawa T, Titani K, WADE R, Malencik D, FISCHER E . Human brain calmodulin: isolation, characterization, and sequence of a half-molecule fragment. Biochemistry. 1981; 20(18):5239-45. DOI: 10.1021/bi00521a022. View

11.

Gopalakrishna R, Anderson W . Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography. Biochem Biophys Res Commun. 1982; 104(2):830-6. DOI: 10.1016/0006-291x(82)90712-4. View

12.

Anderson J, Charbonneau H, Jones H, McCann R, Cormier M . Characterization of the plant nicotinamide adenine dinucleotide kinase activator protein and its identification as calmodulin. Biochemistry. 1980; 19(13):3113-20. DOI: 10.1021/bi00554a043. View

13.

Eldik L, Grossman A, Iverson D, Watterson D . Isolation and characterization of calmodulin from spinach leaves and in vitro translation mixtures. Proc Natl Acad Sci U S A. 1980; 77(4):1912-6. PMC: 348619. DOI: 10.1073/pnas.77.4.1912. View

14.

Thompson M, Brower D, Farrell Jr H . Absence of detectable calmodulin in cows' milk by a modified gel electrophoresis method. J Dairy Sci. 1987; 70(6):1134-9. DOI: 10.3168/jds.S0022-0302(87)80123-6. View

15.

Wang J, Waisman D . Calmodulin and its role in the second-messenger system. Curr Top Cell Regul. 1979; 15:47-107. DOI: 10.1016/b978-0-12-152815-7.50006-5. View

16.

Cornish-Bowden A . Relating proteins by amino acid composition. Methods Enzymol. 1983; 91:60-75. DOI: 10.1016/s0076-6879(83)91011-x. View

17.

Kretsinger R . Crystallographic studies of calmodulin and homologs. Ann N Y Acad Sci. 1980; 356:14-9. DOI: 10.1111/j.1749-6632.1980.tb29594.x. View

18.

Ho C, Teo T, Desai R, Wang J . Catalytic and regulatory properties of two forms of bovine heart cyclic nucleotide phosphodiesterase. Biochim Biophys Acta. 1976; 429(2):461-73. DOI: 10.1016/0005-2744(76)90294-1. View

19.

Muto S, Miyachi S . Properties of a Protein Activator of NAD Kinase from Plants. Plant Physiol. 1977; 59(1):55-60. PMC: 542330. DOI: 10.1104/pp.59.1.55. View

20.

Watterson D, Sharief F, Vanaman T . The complete amino acid sequence of the Ca2+-dependent modulator protein (calmodulin) of bovine brain. J Biol Chem. 1980; 255(3):962-75. View