Molecular Cloning of Complementary DNA Encoding Maize Nitrite Reductase: Molecular Analysis and Nitrate Induction

Overview

Journal Plant Physiol

Specialty Physiology

Date 1988 Nov 1

PMID 16666376

Citations 37

Authors

K Lahners

V Kramer

E Back

L Privalle

S Rothstein

Affiliations

Soon will be listed here.

Abstract

Complementary DNA has been isolated that codes for maize nitrite reductase (NiR) by using the corresponding spinach gene (E Back et al. 1988 Mol Gen Genet 212:20-26) as a heterologous probe. The sequences of the complementary DNAs from the two species are 66% homologous while the deduced amino acid sequences are 86% similar when analogous amino acids are included. A high percentage of the differences in the DNA sequences is due to the extremely strong bias in the corn gene to have a G/C base in the third codon position with 559/569 codons ending in a G or C. Using a hydroponic system, maize seedlings grown in the absence of an exogenous nitrogen source were induced with nitrate or nitrite. Nitrate stimulated a rapid induction of the NiR mRNA in both roots and leaves. There is also a considerable induction of this gene in roots upon the addition of nitrite, although under the conditions used the final mRNA level was not as high as when nitrate was the inducer. There is a small but detectable level of NiR mRNA in leaves prior to induction, but no constitutive NiR mRNA can be seen in the roots. Analysis of genomic DNA supports the notion that there are at least two NiR genes in maize.

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References

Dalling M, Hucklesby D, Hageman R . A Comparison of Nitrite Reductase Enzymes from Green Leaves, Scutella, and Roots of Corn (Zea mays L.). Plant Physiol. 1973; 51(3):481-4. PMC: 366292. DOI: 10.1104/pp.51.3.481. View

Lips S, Roth-Bejerano N . Light and hormones: interchangeability in the induction of nitrate reductase. Science. 1969; 166(3901):109-10. DOI: 10.1126/science.166.3901.109. View

Aparicio P, Knaff D, Malkin R . The role of an iron-sulfur center and siroheme in spinach nitrite reductase. Arch Biochem Biophys. 1975; 169(1):102-7. DOI: 10.1016/0003-9861(75)90321-5. View

Young R, Davis R . Efficient isolation of genes by using antibody probes. Proc Natl Acad Sci U S A. 1983; 80(5):1194-8. PMC: 393560. DOI: 10.1073/pnas.80.5.1194. View

McRee D, Richardson D, Richardson J, Siegel L . The heme and Fe4S4 cluster in the crystallographic structure of Escherichia coli sulfite reductase. J Biol Chem. 1986; 261(22):10277-81. View

Calza R, Huttner E, Vincentz M, Rouze P, Galangau F, Vaucheret H . Cloning of DNA fragments complementary to tobacco nitrate reductase mRNA and encoding epitopes common to the nitrate reductases from higher plants. Mol Gen Genet. 1987; 209(3):552-62. PMC: 7087718. DOI: 10.1007/BF00331162. View

McClure P, Omholt T, Pace G, Bouthyette P . Nitrate-induced changes in protein synthesis and translation of RNA in maize roots. Plant Physiol. 1987; 84(1):52-7. PMC: 1056526. DOI: 10.1104/pp.84.1.52. View

Moore R, Davies M, Oconnell K, Harding E, Wiegand R, Tiemeier D . Cloning and expression of a cDNA encoding a maize glutathione-S-transferase in E. coli. Nucleic Acids Res. 1986; 14(18):7227-35. PMC: 311748. DOI: 10.1093/nar/14.18.7227. View

Baker A, Leaver C . Isolation and sequence analysis of a cDNA encoding the ATP/ADP translocator of Zea mays L. Nucleic Acids Res. 1985; 13(16):5857-67. PMC: 321917. DOI: 10.1093/nar/13.16.5857. View

10.

Heath-Pagliuso S, Huffaker R, Allard R . Inheritance of nitrite reductase and regulation of nitrate reductase, nitrite reductase, and glutamine synthetase isozymes. Plant Physiol. 1984; 76(2):353-8. PMC: 1064291. DOI: 10.1104/pp.76.2.353. View

11.

Murphy M, Siegel L, TOVE S, KAMIN H . Siroheme: a new prosthetic group participating in six-electron reduction reactions catalyzed by both sulfite and nitrite reductases. Proc Natl Acad Sci U S A. 1974; 71(3):612-6. PMC: 388061. DOI: 10.1073/pnas.71.3.612. View

12.

Izui K, Ishijima S, Yamaguchi Y, Katagiri F, Murata T, Shigesada K . Cloning and sequence analysis of cDNA encoding active phosphoenolpyruvate carboxylase of the C4-pathway from maize. Nucleic Acids Res. 1986; 14(4):1615-28. PMC: 339534. DOI: 10.1093/nar/14.4.1615. View

13.

Crawford N, Campbell W, Davis R . Nitrate reductase from squash: cDNA cloning and nitrate regulation. Proc Natl Acad Sci U S A. 1986; 83(21):8073-6. PMC: 386869. DOI: 10.1073/pnas.83.21.8073. View

14.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

15.

Okayama H, Berg P . High-efficiency cloning of full-length cDNA. Mol Cell Biol. 1982; 2(2):161-70. PMC: 369769. DOI: 10.1128/mcb.2.2.161-170.1982. View

16.

Small I, Gray J . Synthesis of wheat leaf nitrite reductase de novo following induction with nitrate and light. Eur J Biochem. 1984; 145(2):291-7. DOI: 10.1111/j.1432-1033.1984.tb08551.x. View

17.

Gupta S, Beevers L . Regulation of nitrite reductase : cell-free translation and processing. Plant Physiol. 1987; 83(4):750-4. PMC: 1056444. DOI: 10.1104/pp.83.4.750. View