Reconstitution of the Light Harvesting Chlorophyll A/b Pigment-Protein Complex into Developing Chloroplast Membranes Using a Dialyzable Detergent

Overview

Journal Plant Physiol

Specialty Physiology

Date 1986 Apr 1

PMID 16664744

Citations 4

Authors

S C Darr

C J Arntzen

Affiliations

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Abstract

Conditions were developed to isolate the light-harvesting chlorophyll-protein complex serving photosystem II (LHC-II) using a dialyzable detergent, octylpolyoxyethylene. This LHC-II was successfully reconstituted into partially developed chloroplast thylakoids of Hordeum vulgare var Morex (barley) seedlings which were deficient in LHC-II. Functional association of LHC-II with the photosystem II (PSII) core complex was measured by two independent functional assays of PSII sensitization by LHC-II. A 3-fold excess of reconstituted LHC-II was required to equal the activity of LHC developing in vivo. We suggest that a linker component may be absent in the partially developed membranes which is required for specific association of the PSII core complex and LHC-II.

Citing Articles

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References

Argyroudi-Akoyunoglou J, Feleki Z, Akoyunoglou G . Formation of two chlorophyll-protein complexes during greening of etiolated bean leaves. Biochem Biophys Res Commun. 1971; 45(3):606-14. DOI: 10.1016/0006-291x(71)90460-8. View

Burke J, Ditto C, Arntzen C . Involvement of the light-harvesting complex in cation regulation of excitation energy distribution in chloroplasts. Arch Biochem Biophys. 1978; 187(1):252-63. DOI: 10.1016/0003-9861(78)90031-0. View

Mullet J, Burke J, Arntzen C . Chlorophyll proteins of photosystem I. Plant Physiol. 1980; 65(5):814-22. PMC: 440430. DOI: 10.1104/pp.65.5.814. View

Schmidt G, Bartlett S, Grossman A, Cashmore A, Chua N . Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol. 1981; 91(2 Pt 1):468-78. PMC: 2111963. DOI: 10.1083/jcb.91.2.468. View

Steinback K, Burke J, Arntzen C . Evidence for the role of surface-exposed segments of the light-harvesting complex in cation-mediated control of chloroplast structure and function. Arch Biochem Biophys. 1979; 195(2):546-57. DOI: 10.1016/0003-9861(79)90381-3. View

McDonnel A, Staehelin L . Adhesion between liposomes mediated by the chlorophyll a/b light-harvesting complex isolated from chloroplast membranes. J Cell Biol. 1980; 84(1):40-56. PMC: 2110522. DOI: 10.1083/jcb.84.1.40. View

Armond P, Staehelin L, Arntzen C . Spatial relationship of photosystem I, photosystem II, and the light-harvesting complex in chloroplast membranes. J Cell Biol. 1977; 73(2):400-18. PMC: 2109912. DOI: 10.1083/jcb.73.2.400. View

Thornber J, Highkin H . Composition of the photosynthetic apparatus of normal barley leaves and a mutant lacking chlorophyll b. Eur J Biochem. 1974; 41(1):109-16. DOI: 10.1111/j.1432-1033.1974.tb03250.x. View

Day D, Ryrie I, Fuad N . Investigations of the role of the main light-harvesting chlorophyll-protein complex in thylakoid membranes. Reconstitution of depleted membranes from intermittent-light-grown plants with the isolated complex. J Cell Biol. 1984; 98(1):163-72. PMC: 2113004. DOI: 10.1083/jcb.98.1.163. View

10.

Glazer A . Comparative biochemistry of photosynthetic light-harvesting systems. Annu Rev Biochem. 1983; 52:125-57. DOI: 10.1146/annurev.bi.52.070183.001013. View

11.

Ryrie I, Anderson J, Goodchild D . The role of the light-harvesting chlorophyll a/b protein complex in chloroplast membrane stacking. Cation-induced aggregation of reconstituted proteoliposomes. Eur J Biochem. 1980; 107(2):345-54. DOI: 10.1111/j.1432-1033.1980.tb06035.x. View

12.

Kyle D, Staehelin L, Arntzen C . Lateral mobility of the light-harvesting complex in chloroplast membranes controls excitation energy distribution in higher plants. Arch Biochem Biophys. 1983; 222(2):527-41. DOI: 10.1016/0003-9861(83)90551-9. View

13.

Mullet J, Burke J, Arntzen C . A developmental study of photosystem I peripheral chlorophyll proteins. Plant Physiol. 1980; 65(5):823-7. PMC: 440431. DOI: 10.1104/pp.65.5.823. View

14.

Mullet J, Arntzen C . Simulation of grana stacking in a model membrane system. Mediation by a purified light-harvesting pigment-protein complex from chloroplasts. Biochim Biophys Acta. 1980; 589(1):100-17. DOI: 10.1016/0005-2728(80)90135-8. View

15.

Sprague S, Camm E, Green B, Staehelin L . Reconstitution of light-harvesting complexes and photosystem II cores into galactolipid and phospholipid liposomes. J Cell Biol. 1985; 100(2):552-7. PMC: 2113455. DOI: 10.1083/jcb.100.2.552. View

16.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

17.

Darr S, Souza Machado V, Arntzen C . Uniparental inheritance of a chloroplast photosystem II polypeptide controlling herbicide binding. Biochim Biophys Acta. 1981; 634(1):219-28. DOI: 10.1016/0005-2728(81)90140-7. View

18.

McCourt P, Browse J, Watson J, Arntzen C, Somerville C . Analysis of Photosynthetic Antenna Function in a Mutant of Arabidopsis thaliana (L.) Lacking trans-Hexadecenoic Acid. Plant Physiol. 1985; 78(4):853-8. PMC: 1064837. DOI: 10.1104/pp.78.4.853. View

19.

BOARDMAN N, Thorne S . Sensitive fluorescence method for the determination of chlorophyll a-chlorophyll b ratios. Biochim Biophys Acta. 1971; 253(1):222-31. DOI: 10.1016/0005-2728(71)90248-9. View

20.

Bennett J . Regulation of photosynthesis by reversible phosphorylation of the light-harvesting chlorophyll a/b protein. Biochem J. 1983; 212(1):1-13. PMC: 1152003. DOI: 10.1042/bj2120001. View