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Arabinogalactan Protein from a Crude Cell Organelle Fraction of Phaseolus Vulgaris L

Overview
Journal Plant Physiol
Specialty Physiology
Date 1981 Oct 1
PMID 16662024
Citations 15
Authors
G J van Holst
F M Klis
P J de Wildt
C A Hazenberg
J Buijs
D Stegwee
Affiliations
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Abstract

Sonication of a crude cell organelle fraction from hypocotyl tissue of dark-grown bean seedlings, and from suspension-cultured cells released a hydroxyproline-containing protein. The purification of this protein is described. It was found to be an arabinogalactan protein composed of 90% carbohydrate and 10% protein. The major sugars are galactose, arabinose, and uronic acids, and the major amino acids are hydroxyproline, serine, and alanine. Its molecular weight was estimated at 1.4 x 10(5) daltons and the isoelectric point at pH 2.3. The molecule is soluble in 5% trichloroacetic acid and can be precipitated with beta-galactosyl Yariv antigen. Pulse-chase experiments indicated that it was a secretory protein. The biosynthesis of arabinogalactan proteins is discussed.

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