Synthesis of Chloroplast Proteins During Germination and Early Development of Cucumber

Overview

Journal Plant Physiol

Specialty Physiology

Date 1981 Jun 1

PMID 16661815

Citations 20

Authors

R Walden

C J Leaver

Affiliations

Soon will be listed here.

Abstract

Cell-free protein synthesizing systems have been used to study the developmental changes in the synthesis of chloroplast proteins in the cotyledons of cucumber seedlings grown in the light or in the dark. Escherichia coli and wheat germ in vitro protein synthesizing systems have been used to assay the changes in the levels of the mRNA's coding for ribulose 1,5-bisphosphate carboxylase (RuBPCase). The large subunit of cucumber RuBPCase has been identified among the translation products of the E. coli system. The wheat germ system translates the cucumber mRNA coding for the small subunit of RuBPCase to produce a 25,000 molecular weight precursor polypeptide. Plastids isolated from light-grown cotyledons were used to study developmental changes in their capacity to synthesize protein. The data obtained indicate that in the light there is an initial 48-hour period of accumulation of the mRNA's coding for the large and small subunits of RuBPCase, coupled with an increase in the capacity of the isolated plastids to synthesize protein. This is followed by a decline. This decline is not reflected in the accumulation of RuBPCase in the cotyledons which remains constant over the period of study.

Citing Articles

The major light-harvesting complex of Photosystem II: aspects of its molecular and cell biology.

Chitnis P, Thornber J Photosynth Res. 2014; 16(1-2):41-63.

PMID: 24430991 DOI: 10.1007/BF00039485.

Protein synthesis by isolated chloroplasts.

Gnanam A, Subbaiah C, Mannan R Photosynth Res. 2014; 19(1-2):129-52.

PMID: 24425371 DOI: 10.1007/BF00114572.

Phytochrome regulation of mRNA levels of ribulose-1,5-bisphosphate carboxylase in etiolated rye seedlings (Secale cereale).

Ernst D, Pfeiffer F, Schefbeck K, Weyrauch C, Oesterhelt D Plant Mol Biol. 2013; 10(1):21-33.

PMID: 24277460 DOI: 10.1007/BF00014183.

Gene expression in cytokinin-and light-mediated plastogenesis of Cucurbita cotyledons: ribulose-1,5-bisphosphate carboxylase/oxygenase.

Lerbs S, Lerbs W, Klyachko N, Romanko E, Kulaeva O, Wollgiehn R Planta. 2013; 162(4):289-98.

PMID: 24253161 DOI: 10.1007/BF00396739.

Expression of two nuclear genes encoding chloroplast proteins during early development of cucumber seedlings.

Greenland A, Thomas M, Walden R Planta. 2013; 170(1):99-110.

PMID: 24232847 DOI: 10.1007/BF00392386.

References

Bottomley W, Higgins T, Whitfeld P . Differential recognition of chloroplast and cytoplasmic messenger RNA by 70S and 80S ribosomal systems. FEBS Lett. 1976; 63(1):120-4. DOI: 10.1016/0014-5793(76)80207-4. View

Hartley M, Wheeler A, Ellis R . Protein synthesis in chloroplasts. V. Translation of messenger RNA for the large subunit of fraction I protein in a heterologous cell-free system. J Mol Biol. 1975; 91(1):67-77. DOI: 10.1016/0022-2836(75)90372-1. View

Dockerty A, Lord J, Merrett M . Development of ribulose-1,5-diphosphate carboxylase in castor bean cotyledons. Plant Physiol. 1977; 59(6):1125-7. PMC: 542519. DOI: 10.1104/pp.59.6.1125. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Kessler S . Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975; 115(6):1617-24. View

Silverthorne J, Ellis R . Protein synthesis in chloroplasts. VIII. Differential synthesis of chloroplast proteins during spinach leaf development. Biochim Biophys Acta. 1980; 607(2):319-30. DOI: 10.1016/0005-2787(80)90084-2. View

Leaver C, Ingle J . The molecular integrity of chloroplast ribosomal ribonucleic acid. Biochem J. 1971; 123(2):235-43. PMC: 1176928. DOI: 10.1042/bj1230235. View

Dobberstein B, Blobel G, Chua N . In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1977; 74(3):1082-5. PMC: 430598. DOI: 10.1073/pnas.74.3.1082. View

Siddell S, Ellis R . Protein synthesis in chloroplasts. Characteristics and products of protein synthesis in vitro in etioplasts and developing chloroplasts from pea leaves. Biochem J. 1975; 146(3):675-85. PMC: 1165358. DOI: 10.1042/bj1460675. View

10.

Mendiola-Morgenthaler L, Morgenthaler J, Price C . Synthesis of coupling factor CF1 protein by isolated spinach chloroplasts. FEBS Lett. 1976; 62(1):96-100. DOI: 10.1016/0014-5793(76)80025-7. View

11.

Marcu K, Dudock B . Characterization of a highly efficient protein synthesizing system derived from commercial wheat germ. Nucleic Acids Res. 1974; 1(11):1385-97. PMC: 343419. DOI: 10.1093/nar/1.11.1385. View

12.

Huisman J, Moorman A, Verkley F . In vitro synthesis of chloroplast ferredoxin as a high molecular weight precursor in a cell-free protein synthesizing system from wheat germs. Biochem Biophys Res Commun. 1978; 82(4):1121-31. DOI: 10.1016/0006-291x(78)90303-0. View

13.

Bedbrook J, Link G, Coen D, Bogorad L . Maize plastid gene expressed during photoregulated development. Proc Natl Acad Sci U S A. 1978; 75(7):3060-4. PMC: 392713. DOI: 10.1073/pnas.75.7.3060. View

14.

Grebanier A, Steinback K, Bogorad L . Comparison of the Molecular Weights of Proteins Synthesized by Isolated Chloroplasts with Those Which Appear during Greening in Zea mays. Plant Physiol. 1979; 63(3):436-9. PMC: 542846. DOI: 10.1104/pp.63.3.436. View

15.

Apel K, Kloppstech K . The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur J Biochem. 1978; 85(2):581-8. DOI: 10.1111/j.1432-1033.1978.tb12273.x. View

16.

Aviv H, Leder P . Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972; 69(6):1408-12. PMC: 426713. DOI: 10.1073/pnas.69.6.1408. View

17.

Bottomley W, Spencer D, Whitfeld P . Protein synthesis in isolated spinach chloroplasts: comparison of light-driven and ATP-driven synthesis. Arch Biochem Biophys. 1974; 164(1):106-17. DOI: 10.1016/0003-9861(74)90012-5. View

18.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

19.

Chua N, Schmidt G . Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978; 75(12):6110-4. PMC: 393128. DOI: 10.1073/pnas.75.12.6110. View

20.

Arnon D . COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949; 24(1):1-15. PMC: 437905. DOI: 10.1104/pp.24.1.1. View