Article: Cell Wall Metabolism in Ripening Fruit: II. CHANGES IN CARBOHYDRATE-DEGRADING ENZYMES IN RIPENING ;BARTLETT' PEARS

Mature ;Bartlett' pear (Pyrus communis) fruits were ripened at 20 C. Fruits at different stages of ripeness were homogenized, and extracts of the low speed pellet (crude cell wall) were prepared. These extracts contained polygalacturonase, pectin esterase, and activity against seven p-nitrophenyl glycoside substrates. Polygalacturonase, alpha-galactosidase, and alpha-mannosidase increased in activity as the fruit ripened. Cellulase and activities against pear wall xylan and arabinan were absent from the extracts.

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References

1.

Keegstra K, Talmadge K, Bauer W, Albersheim P . The Structure of Plant Cell Walls: III. A Model of the Walls of Suspension-cultured Sycamore Cells Based on the Interconnections of the Macromolecular Components. Plant Physiol. 1973; 51(1):188-97. PMC: 367377. DOI: 10.1104/pp.51.1.188. View

2.

Jacobs M, Ray P . Promotion of Xyloglucan Metabolism by Acid pH. Plant Physiol. 1975; 56(3):373-6. PMC: 541826. DOI: 10.1104/pp.56.3.373. View

3.

Chester M, Hultberg B, OCKERMAN P . The common identity of five glycosidases in human liver. Biochim Biophys Acta. 1976; 429(2):517-26. DOI: 10.1016/0005-2744(76)90299-0. View

4.

Maxie E, Eaks I, Sommer N, Rae H, El-Batal S . Effect of Gamma Radiation on Rate of Ethylene and Carbon Dioxide Evolution by Lemon Fruit. Plant Physiol. 1965; 40(3):407-9. PMC: 550306. DOI: 10.1104/pp.40.3.407. View

5.

Hobson G . Pectinesterase in normal and abnormal tomato fruit. Biochem J. 1963; 86:358-65. PMC: 1201761. DOI: 10.1042/bj0860358. View