Starch-synthesizing Enzymes in the Endosperm and Pollen of Maize

Overview

Journal Plant Physiol

Specialty Physiology

Date 1979 Feb 1

PMID 16660720

Citations 10

Authors

W H Bryce

O E Nelson

Affiliations

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Abstract

Two mutations, amylose-extender and waxy, which affect the proportion of amylose and amylopectin of starch synthesized in the endosperm of maize (Zea mays L.) seeds, are also expressed in the pollen. However, most mutations that affect starch synthesis in the maize endosperm are not expressed in the pollen. In an attempt to understand the nonconcordance between the endosperm and pollen, extracts of mature pollen grains were assayed for a number of the enzymes possibly implicated in starch synthesis in the endosperm. Sucrose synthetase (sucrose-UDP glucosyl transferase, EC 2.4.1.13) activity was not detectable in either mature or immature pollen grains of nonmutant maize, but both bound and soluble invertase (EC 3.2.1.26) exhibited much greater specific activity (per milligram protein) in pollen extracts than in 22-day-old endosperm extracts. Phosphorylase (EC 2.4.1.1) activity was also higher in pollen than in endosperm extracts. ADP-Glucose pyrophosphorylase (EC 2.7.7.27) activity was much lower in pollen than endosperm extracts, but mutations that drastically reduced ADP-glucose pyrophosphorylase activity in the endosperm (brittle-2 and shrunken-2) did not markedly affect enzymic activity in the pollen. Specific activities of other enzymes implicated in starch synthesis were similar in endosperm and pollen extracts.

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References

Hannah L, Nelson O . Characterization of adenosine diphosphate glucose pyrophosphorylases from developing maize seeds. Plant Physiol. 1975; 55(2):297-302. PMC: 541603. DOI: 10.1104/pp.55.2.297. View

Brink R . Mendelian Ratios and the Gametophyte Generation in Angiosperms. Genetics. 1925; 10(4):359-94. PMC: 1200867. DOI: 10.1093/genetics/10.4.359. View

Laughnan J . The Effect of the sh(2) Factor on Carbohydrate Reserves in the Mature Endosperm of Maize. Genetics. 1953; 38(5):485-99. PMC: 1209620. DOI: 10.1093/genetics/38.5.485. View

HASSID W, Neufeld E, Feingold D . SUGAR NUCLEOTIDES IN THE INTERCONVERSION OF CARBOHYDRATES IN HIGHER PLANTS. Proc Natl Acad Sci U S A. 1959; 45(7):905-15. PMC: 222664. DOI: 10.1073/pnas.45.7.905. View

Hannah L, Nelson Jr O . Characterization of ADP-glucose pyrophosphorylase from shrunken-2 and brittle-2 mutants of maize. Biochem Genet. 1976; 14(7-8):547-60. DOI: 10.1007/BF00485834. View

Nelson O, Tsai C . GLUCOSE TRANSFER FROM ADENOSINE DIPHOSPHATE-GLUCOSE TO STARCH IN PREPARATIONS OF WAXY SEEDS. Science. 1964; 145(3637):1194-5. DOI: 10.1126/science.145.3637.1194. View

Burr B, Nelson O . The phosphorylases of developing maize seeds. Ann N Y Acad Sci. 1973; 210:129-38. DOI: 10.1111/j.1749-6632.1973.tb47567.x. View

Ozbun J, Hawker J, Preiss J . Adenosine diphosphoglucose-starch glucosyltransferases from developing kernels of waxy maize. Plant Physiol. 1971; 48(6):765-9. PMC: 396944. DOI: 10.1104/pp.48.6.765. View

Nelson O, Rines H . The enzymatic deficiency in the waxy mutant of maize. Biochem Biophys Res Commun. 1962; 9:297-300. DOI: 10.1016/0006-291x(62)90043-8. View

10.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

11.

Burr B, Nelson O . Maize alpha-glucan phosphorylase. Eur J Biochem. 1975; 56(2):539-46. DOI: 10.1111/j.1432-1033.1975.tb02260.x. View

12.

Akatsuka T, Nelson O . Starch granule-bound adenosine diphosphate glucose-starch glucosyltransferases of maize seeds. J Biol Chem. 1966; 241(10):2280-5. View

13.

Tsai C, Nelson O . Starch-deficient maize mutant lacking adenosine dephosphate glucose pyrophosphorylase activity. Science. 1966; 151(3708):341-3. DOI: 10.1126/science.151.3708.341. View

14.

Tsai C, Nelson O . Phosphorylases I and II of Maize Endosperm. Plant Physiol. 1968; 43(1):103-12. PMC: 396019. DOI: 10.1104/pp.43.1.103. View

15.

Dickinson D, Preiss J . Presence of ADP-Glucose Pyrophosphorylase in Shrunken-2 and Brittle-2 Mutants of Maize Endosperm. Plant Physiol. 1969; 44(7):1058-62. PMC: 396214. DOI: 10.1104/pp.44.7.1058. View

16.

Ozbun J, Hawker J, Greenberg E, Lammel C, Preiss J . Starch Synthetase, Phosphorylase, ADPglucose Pyrophosphorylase, and UDPglucose Pyrophosphorylase in Developing Maize Kernels. Plant Physiol. 1973; 51(1):1-5. PMC: 367346. DOI: 10.1104/pp.51.1.1. View

17.

Tsai C, Salamini F, Nelson O . Enzymes of carbohydrate metabolism in the developing endosperm of maize. Plant Physiol. 1970; 46(2):299-306. PMC: 396583. DOI: 10.1104/pp.46.2.299. View

18.

Preiss J, Lammel C, Sabraw A . A unique adenosine diphosphoglucose pyrophosphorylase associated with maize embryo tissue. Plant Physiol. 1971; 47(1):104-8. PMC: 365820. DOI: 10.1104/pp.47.1.104. View

19.

Chourey P, Nelson O . The enzymatic deficiency conditioned by the shrunken-1 mutations in maize. Biochem Genet. 1976; 14(11-12):1041-55. DOI: 10.1007/BF00485135. View

20.

Dickinson D, Preiss J . ADP glucose pyrophosphorylase from maize endosperm. Arch Biochem Biophys. 1969; 130(1):119-28. DOI: 10.1016/0003-9861(69)90017-4. View