Presence of the Cyanogenic Glucoside Dhurrin in Isolated Vacuoles from Sorghum

Overview

Journal Plant Physiol

Specialty Physiology

Date 1978 Feb 1

PMID 16660251

Citations 35

Authors

J A Saunders

E E Conn

Affiliations

Soon will be listed here.

Abstract

Large numbers of vacuoles (10(6)-10(7)) have been isolated from Sorghum bicolor protoplasts and analyzed for the cyanogenic glucoside dhurrin. Leaves from light-grown seedlings were incubated for 4 hours in 1.5% cellulysin and 0.5% macerase to yield mesophyll protoplasts which then were recovered by centrifugation, quantitated by a hemocytometer, and assayed for cyanogenic glucosides. Mature vacuoles, released from the protoplasts by osmotic shock, were purified on a discontinuous Ficoll gradient and monitored for intactness by their ability to maintain a slightly acid interior while suspended in an alkaline buffer as indicated by neutral red stain. Cyanide analysis of the protoplasts and the vacuoles obtained there from yielded equivalent values of 11 mumoles of cyanogenic glucoside dhurrin per 10(7) protoplasts or 10(7) vacuoles. This work supports an earlier study from this laboratory which demonstrated that the vacuole is the site of accumulation of the cyanogenic glucoside in Sorghum.

Citing Articles

Regulation of dhurrin pathway gene expression during Sorghum bicolor development.

Gleadow R, McKinley B, Blomstedt C, Lamb A, Moller B, Mullet J Planta. 2021; 254(6):119.

PMID: 34762174 PMC: 8585852. DOI: 10.1007/s00425-021-03774-2.

Molecular identification and functional characterization of a cyanogenic glucosyltransferase from flax (Linum unsitatissimum).

Kazachkov M, Li Q, Shen W, Wang L, Gao P, Xiang D PLoS One. 2020; 15(2):e0227840.

PMID: 32023283 PMC: 7001965. DOI: 10.1371/journal.pone.0227840.

Role of cyanogenic glycosides in the seeds of wild lima bean, Phaseolus lunatus: defense, plant nutrition or both?.

Cuny M, La Forgia D, Desurmont G, Glauser G, Benrey B Planta. 2019; 250(4):1281-1292.

PMID: 31240396 DOI: 10.1007/s00425-019-03221-3.

Label-free Raman hyperspectral imaging analysis localizes the cyanogenic glucoside dhurrin to the cytoplasm in sorghum cells.

Heraud P, Cowan M, Marzec K, Moller B, Blomstedt C, Gleadow R Sci Rep. 2018; 8(1):2691.

PMID: 29426935 PMC: 5807435. DOI: 10.1038/s41598-018-20928-7.

Dhurrin metabolism in the developing grain of Sorghum bicolor (L.) Moench investigated by metabolite profiling and novel clustering analyses of time-resolved transcriptomic data.

Nielsen L, Stuart P, Picmanova M, Rasmussen S, Olsen C, Harholt J BMC Genomics. 2016; 17(1):1021.

PMID: 27964718 PMC: 5154151. DOI: 10.1186/s12864-016-3360-4.

References

Indge K . The isolation and properties of the yeast cell vacuole. J Gen Microbiol. 1968; 51(3):441-6. DOI: 10.1099/00221287-51-3-441. View

Butcher H, Wagner G, Siegelman H . Localization of Acid hydrolases in protoplasts: examination of the proposed lysosomal function of the mature vacuole. Plant Physiol. 1977; 59(6):1098-103. PMC: 542514. DOI: 10.1104/pp.59.6.1098. View

Beier H, Bruening G . The use of an abrasive in the isolation of cowpea leaf protoplasts which support the multiplication of cowpea mosaic virus. Virology. 1975; 64(1):272-6. DOI: 10.1016/0042-6822(75)90099-9. View

Nakamura K . The isolation of vacuoles from Candida utilis. Prep Biochem. 1973; 3(6):553-61. DOI: 10.1080/00327487308061537. View

Leigh R, Branton D . Isolation of Vacuoles from Root Storage Tissue of Beta vulgaris L. Plant Physiol. 1976; 58(5):656-62. PMC: 542277. DOI: 10.1104/pp.58.5.656. View