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Ammonia Regulation of Carbon Metabolism in Photosynthesizing Leaf Discs

Overview
Journal Plant Physiol
Specialty Physiology
Date 1977 Nov 1
PMID 16660175
Citations 18
Authors
S G Platt
Affiliations
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Abstract

Alfalfa (Medicago sativa L., var. El Unico) leaf discs, floating on buffer containing NH(4)Cl and photosynthesizing with (14)CO(2), produced more labeled amino acid and less sucrose than did control discs (no added NH(4)Cl). The level of pyruvate increased and that of phosphoenolpyruvate decreased. These and other changes in levels of labeled compounds led us to conclude that pyruvate kinase was activated by ammonia, resulting in increased transfer of photosynthetically incorporated carbon to synthesis of amino acid skeletons at the expense of sucrose synthesis. Carbon flow through enzymes catalyzing the anaplerotic reactions was apparently stimulated.

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References
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Platt S, Bassham J . Separation of 14 C-labeled glycolate pathway metabolites from higher plant photosynthate. J Chromatogr. 1977; 133(2):396-401. DOI: 10.1016/s0021-9673(00)83504-9. View
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Platt S, Plaut Z, Bassham J . Steady-state photosynthesis in alfalfa leaflets: effects of carbon dioxide concentration. Plant Physiol. 1977; 60(2):230-4. PMC: 542586. DOI: 10.1104/pp.60.2.230. View
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Platt S, Plaut Z, Bassham J . Analysis of steady state photosynthesis in alfalfa leaves. Plant Physiol. 1976; 57(1):69-73. PMC: 541965. DOI: 10.1104/pp.57.1.69. View