Comparative Studies of Glyoxysomes from Various Fatty Seedlings

Overview

Journal Plant Physiol

Specialty Physiology

Date 1975 May 1

PMID 16659183

Citations 17

Authors

A H Huang

Affiliations

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Abstract

The separation of various organelles from cotton cotyledon (Gossypium hirsutum L.), cucumber cotyledon (Cucumis sativus L.), peanut cotyledon (Archis hypogaea L.), pine megagametophyte (Pinus ponderosa Laws), and watermelon cotyledon (Citrullus vulgaris Schrad.) by sucrose density gradient centrifugation was found to be similar to that described for castor bean endosperm (Ricinus communis L.). Equilibrium densities were 1.12 to 1.13 g cm(3) for endoplasmic reticulum, 1.17 to 1.19 g/cm(3) for mitochondria, and 1.25 g/cm(3) for glyoxysomes. Isolated glyoxysomes from different fatty seedlings have striking similar specific activities of individual enzymes. The only exception is alkaline lipase activity which, when assayed with an artificial substrate, varies some 10-fold in glyoxysomes from different fatty seedlings. The properties of individual enzymes in glyoxysomes from different fatty seedlings are qualitatively similar as regard to sub-organelle localization and behavior in the presence of KCl and Triton X-100. In pine megagametophyte, the glyoxysomes and not the mitochondria are the intracellular site for the breakdown of stored lipid.

Citing Articles

Phytochrome-mediated transformation of glyoxysomes into peroxisomes in the cotyledons of mustard (Sinapis alba L.) seedlings.

Schopfer P, Bajracharya D, Bergfeld R, Falk H Planta. 2014; 133(1):73-80.

PMID: 24425181 DOI: 10.1007/BF00386008.

Developmental studies of NAD-malate dehydrogenase isozymes in the cotyledon of cucumber seedlings grown in darkness and in light.

Liu K, Huang A Planta. 2014; 131(3):279-84.

PMID: 24424831 DOI: 10.1007/BF00385427.

Effect of light on the development of glyoxysomal functions in the cotyledons of mustard (Sinapis alba L.) seedlings.

Bajracharya D, Schopfer P Planta. 2013; 145(2):181-6.

PMID: 24317674 DOI: 10.1007/BF00388715.

Fatty acid β-oxidation and glyoxylate cycle enzyme activities of induced glyoxysomes from anise suspension cultures.

Lutzenberger A, Theimer R Plant Cell Rep. 2013; 2(3):160-3.

PMID: 24257989 DOI: 10.1007/BF00269344.

Partial purification and characterization of the mitochondrial and peroxisomal isozymes of enoyl-coenzyme a hydratase from germinating pea seedlings.

Miernyk J, Thomas D, Wood C Plant Physiol. 1991; 95(2):564-9.

PMID: 16668020 PMC: 1077569. DOI: 10.1104/pp.95.2.564.

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