Soybean Polyamines: Separation and Characterization of Cadaverine

Cadaverine in soybeans was separated by ion exchange chromatography from other polyamines previously identified. Identification of cadaverine was based on ion exchange separation, thin layer chromatography, paper electrophoresis, mass and nuclear magnetic resonance spectral analyses. Since the molecules of putrescine and cadaverine are so similar, separation and identification of the two components are difficult. Their R(F) values on thin layer chromatography are close, although cadaverine produces a bluish purple color when sprayed with ninhydrin reagent, while putrescine forms a purple color. Separation likewise is poor by paper electrophoresis, gas chromatography, and gel filtration. The mass spectra of cadaverine and putrescine have m/e peaks at 30, 43, 45, 56, 73, 85, 102 and 30, 43, 59, 71, 88, respectively. The m/e peaks differentiate one compound from the other. Nuclear magnetic resonance spectra and their integration curves show that cadaverine contains two types of methylene protons (10 total) in 3:2 ratio while putrescine produces two types (8 total) in 1:1 ratio. Polyamines occur at levels of micrograms per gram of soybeans with spermidine present in the largest quantity.