Synthesis and Turnover of Nitrate Reductase in Corn Roots

Overview

Journal Plant Physiol

Specialty Physiology

Date 1972 Dec 1

PMID 16658236

Citations 39

Authors

A Oaks

W Wallace

D Stevens

Affiliations

Soon will be listed here.

Abstract

THE INDUCTION AND REINDUCTION OF NITRATE REDUCTASE IN ROOT TIP OR MATURE ROOT SECTIONS SHOW ESSENTIALLY A SIMILAR PATTERN: a lag, a period of rapid increase in enzyme activity and finally a period of relatively minor change. Both inductions are sensitive to 6-methylpurine and cycloheximide. Kinetic studies with 6-methylpurine suggest that the half-life of the messenger RNA for nitrate reductase in both sections is about 20 minutes. The rate of decay of nitrate reductase activity induced by transfer to a nitrate-free medium is slower in root tips (t(1/2) = 3 hours) than in mature root sections (t(1/2) = 2 hours). The enzyme from mature root sections is also less stable to mild heat treatments (27 C; 40 C) than the enzyme from root tip sections. The results indicate that factors regulating enzyme turnover show important changes as root cells mature and may be significant in determining steady state levels of the enzyme.

Citing Articles

Nitrate deposition in northern hardwood forests and the nitrogen metabolism of Acer saccharum marsh.

Rothstein D, Zak D, Pregitzer K Oecologia. 2017; 108(2):338-344.

PMID: 28307847 DOI: 10.1007/BF00334659.

Ammonium repression of nitrate reductase formation in Lemna minor L.

Orebamjo T, Stewart G Planta. 2014; 122(1):27-36.

PMID: 24435918 DOI: 10.1007/BF00385401.

Photosynthesis and the induction of nitrate reductase and nitrite reductase in bean leaves.

Sluiters-Scholten C Planta. 2014; 123(2):175-84.

PMID: 24435084 DOI: 10.1007/BF00383866.

Leaf development and the role of NADP-malate dehydrogenase in C3 plants.

Vivekanandan M, Edwards G Photosynth Res. 2014; 14(2):125-35.

PMID: 24430666 DOI: 10.1007/BF00032317.

Nitrate reductase activity in Paul's scarlet rose suspension cultures and the differential role of nitrate and molybdenum in induction.

Jones R, Abbott A, Hewitt E, Best G, Watson E Planta. 2014; 141(2):183-9.

PMID: 24414775 DOI: 10.1007/BF00387887.

References

Warner R, Hageman R, Dudley J, Lambert R . Inheritance of nitrate reductase activity in Zea mays L. Proc Natl Acad Sci U S A. 1969; 62(3):785-92. PMC: 223667. DOI: 10.1073/pnas.62.3.785. View

Martin Jr D, Tomkins G, Granner D . Synthesis and induction of tyrosine aminotransferase in synchronized hepatoma cells in culture. Proc Natl Acad Sci U S A. 1969; 62(1):248-55. PMC: 285980. DOI: 10.1073/pnas.62.1.248. View

ERICKSON R, GODDARD D . An analysis of root growth in cellular and biochemical terms. Growth. 1951; (Suppl 10):89-116. View

Sanderson G, Cocking E . Enzymic Assimilation of Nitrate in Tomato Plants. I. Reduction of Nitrate to Nitrite. Plant Physiol. 1964; 39(3):416-22. PMC: 550096. DOI: 10.1104/pp.39.3.416. View

SIPPEL A, Hartmann G . Mode of action of rafamycin on the RNA polymerase reaction. Biochim Biophys Acta. 1968; 157(1):218-9. DOI: 10.1016/0005-2787(68)90286-4. View

Oaks A . Transport of amino acids to the maize root. Plant Physiol. 1966; 41(1):173-80. PMC: 1086315. DOI: 10.1104/pp.41.1.173. View

Travis R, JORDAN W, Huffaker R . Evidence for an Inactivating System of Nitrate Reductase in Hordeum vulgare L. during Darkness That Requires Protein Synthesis. Plant Physiol. 1969; 44(8):1150-6. PMC: 396231. DOI: 10.1104/pp.44.8.1150. View

Cove D, Pateman J . Autoregulation of the synthesis of nitrate reductase in Aspergillus nidulans. J Bacteriol. 1969; 97(3):1374-8. PMC: 249857. DOI: 10.1128/jb.97.3.1374-1378.1969. View

Oaks A, Beevers H . The Requirement for Organic Nitrogen in Zea mays Embryos. Plant Physiol. 1964; 39(1):37-43. PMC: 550023. DOI: 10.1104/pp.39.1.37. View

10.

Schimke R, SWEENEY E, Berlin C . THE ROLES OF SYNTHESIS AND DEGRADATION IN THE CONTROL OF RAT LIVER TRYPTOPHAN PYRROLASE. J Biol Chem. 1965; 240:322-31. View

11.

Heimer Y, Filner P . Regulation of the nitrate assimilation pathway of cultured tobacco cells. II. Properties of a variant cell line. Biochim Biophys Acta. 1970; 215(1):152-65. DOI: 10.1016/0304-4165(70)90398-3. View

12.

SORGER G . Nitrate reductase electron transport systems in mutant and in wild-type strains of Neurospora. Biochim Biophys Acta. 1966; 118(3):484-94. DOI: 10.1016/s0926-6593(66)80091-7. View

13.

Schrader L, Ritenour G, Eilrich G, Hageman R . Some characteristics of nitrate reductase from higher plants. Plant Physiol. 1968; 43(6):930-40. PMC: 1086950. DOI: 10.1104/pp.43.6.930. View

14.

Jensen W . A morphological and biochemical analysis of the early phases of cellular growth in the root tip of Vicia faba. Exp Cell Res. 1955; 8(3):506-22. DOI: 10.1016/0014-4827(55)90127-0. View

15.

Dezelee S, Sentenac A, FROMAGEOT P . Study on yeast RNA polymerase. Effect of alpha-amanitan and rifampicin. FEBS Lett. 1970; 7(3):220-222. DOI: 10.1016/0014-5793(70)80165-x. View

16.

Tomkins G, Gelehrter T, Granner D, Martin Jr D, Samuels H, Thompson E . Control of specific gene expression in higher organisms. Expression of mammalian genes may be controlled by repressors acting on the translation of messenger RNA. Science. 1969; 166(3912):1474-80. DOI: 10.1126/science.166.3912.1474. View

17.

Filner P . Regulation of nitrate reductase in cultured tobacco cells. Biochim Biophys Acta. 1966; 118(2):299-310. DOI: 10.1016/s0926-6593(66)80038-3. View

18.

Lewis C, Fincham J . Regulation of nitrate reductase in the basidiomycete Ustilago maydis. J Bacteriol. 1970; 103(1):55-61. PMC: 248038. DOI: 10.1128/jb.103.1.55-61.1970. View

19.

Filner P, Varner J, Wray J . Environmental or developmental changes cause many enzyme activities of higher plants to rise or fall. Science. 1969; 165(3891):358-67. DOI: 10.1126/science.165.3891.358. View

20.

Zielke H, Filner P . Synthesis and turnover of nitrate reductase induced by nitrate in cultured tobacco cells. J Biol Chem. 1971; 246(6):1772-9. View