The Phylogenetic Position of Ixodes Stilesi Neumann, 1911 (Acari: Ixodidae): Morphological and Preliminary Molecular Evidences from 16S RDNA Sequences

Overview

Journal Syst Parasitol

Specialty Parasitology

Date 2006 Apr 14

PMID 16612654

Citations 14

Authors

Alberto A Guglielmone

Jose M Venzal

Daniel Gonzalez-Acuna

Santiago Nava

Ana Hinojosa

Atilio J Mangold

Affiliations

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Abstract

The female of Ixodes stilesi Neumann, 1911 (Acari Ixodidae) is redescribed and the male and nymph are described from specimens collected from Pudu puda (Molina) (Artiodactyla: Cervidae) in Chile. Both sexes of I. stilesi have characteristics of the subgenera Ixodes Latreille, 1795 and Ixodiopsis Filippova, 1957. The females of I. stilesi are peculiar in having the combination of the sinuous scutum outline, rounded porose areas with distinct borders separated by the width of one area, slender and long palpi, and two subequal spurs on coxa I. The male is unique in having a combination of a posteriorly wrinkled marginal folder, a basis capituli longer than wide, a non-crenulate hypostome toothed portion, two spurs on coxa II to IV and the presence of a pseudoscutum. The nymph of I. stilesi has blunt anterior and posterior processes on palpal article I (characteristics of the subgenus Ixodiopsis and some Pholeoixodes Schulze, 1942) and a wing-shaped basis capituli with a prominent triangular cornua. Phylogenetic analyses based on 16S mitochondrial rDNA sequences of 12 Neotropical and two Australian Ixodes species, plus three argasids, were carried out to clarify the position of I. stilesi. The results of phylogenetic analyses and morphological characters indicate a close relationships between I. stilesi and two other Neotropical species of uncertain subgeneric status, I. neuquenensis Ringuelet, 1947 and I. sigelos Keirans, Clifford & Corwin, 1976.

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References

Xu G, Fang Q, Keirans J, Durden L . Molecular phylogenetic analyses indicate that the Ixodes ricinus complex is a paraphyletic group. J Parasitol. 2003; 89(3):452-7. DOI: 10.1645/0022-3395(2003)089[0452:MPAITT]2.0.CO;2. View

KOHLS G . Ixodes taglei n. sp. (Acarina: Ixodidae) a parasite of the deer, Pudu pudu (Wol.), in Chile. J Med Entomol. 1969; 6(3):280-3. DOI: 10.1093/jmedent/6.3.280. View

KOHLS G . Eight new species of Ixodes from Central and South America (Acarina: Ixodidae). J Parasitol. 1956; 42(6):636-49. View

Mangold A, Bargues M, Mas-Coma S . 18S rRNA gene sequences and phylogenetic relationships of European hard-tick species (Acari: Ixodidae). Parasitol Res. 1998; 84(1):31-7. DOI: 10.1007/s004360050352. View

Barker S, Murrell A . Systematics and evolution of ticks with a list of valid genus and species names. Parasitology. 2005; 129 Suppl:S15-36. DOI: 10.1017/s0031182004005207. View

Osorio G . [Search for the spirochete Borrelia burgdorferi sensu lato by polymerase chain reaction in wild Chilean ticks]. Rev Med Chil. 2001; 129(3):270-6. View

Guglielmone A, Venzal J, Amico G, Mangold A, Keirans J . Description of the nymph and larva and redescription of the female of Ixodes neuquenensis Ringuelet, 1947 (Acari: Ixodidae), a parasite of the endangered Neotropical marsupial Dromiciops gliroides Thomas (Microbiotheria: Microbiotheriidae). Syst Parasitol. 2004; 57(3):211-9. DOI: 10.1023/B:SYPA.0000019082.96187.9c. View

Keirans J, CLIFFORD C, Corwin D . Ixodes sigelos, n. sp. (Acarina : Ixodidae), a parasite of rodents in Chile, with a method for preparing ticks for examination by scanning electron microscopy. Acarologia. 1976; 18(2):217-25. View

Corwin D, CLIFFORD C, Keirans J . An improved method for cleaning and preparing ticks for examination with the scanning electron microscope. J Med Entomol. 1979; 16(4):352-3. DOI: 10.1093/jmedent/16.4.352. View

10.

Marques S, Barros-Battesti D, Onofrio V, Famadas K, Faccini J, Keirans J . Redescription of larva, nymph and adults of Ixodes (I .) loricatus Neumann, 1899 (Acari: Ixodidae) based on light and scanning electron microscopy. Syst Parasitol. 2004; 59(2):135-46. DOI: 10.1023/B:SYPA.0000044430.05551.44. View

11.

Kumar S, Tamura K, Nei M . MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform. 2004; 5(2):150-63. DOI: 10.1093/bib/5.2.150. View

12.

KOHLS G, CLIFFORD C . Ixodes (Haemixodes) uruguayensis, new subgenus, new species (Acarina: Ixodidae) from small rodents in Uruguay. Ann Entomol Soc Am. 1967; 60(2):391-4. DOI: 10.1093/aesa/60.2.391. View

13.

Horak I, Camicas J, Keirans J . The Argasidae, Ixodidae and Nuttalliellidae (Acari: Ixodida): a world list of valid tick names. Exp Appl Acarol. 2003; 28(1-4):27-54. DOI: 10.1023/a:1025381712339. View

14.

Black 4th W, Piesman J . Phylogeny of hard- and soft-tick taxa (Acari: Ixodida) based on mitochondrial 16S rDNA sequences. Proc Natl Acad Sci U S A. 1994; 91(21):10034-8. PMC: 44952. DOI: 10.1073/pnas.91.21.10034. View

15.

Venzal J, Castro O, Cabrera P, de Souza C, Fregueiro G, Barros-Battesti D . Ixodes (Haemixodes) longiscutatum Boero (new status) and I. (H.) uruguayensis Kohls & Clifford, a new synonym of I. (H.) longiscutatum (Acari: Ixodidae). Mem Inst Oswaldo Cruz. 2002; 96(8):1121-2. DOI: 10.1590/s0074-02762001000800015. View