Alpha 2.0
Login Register
» Articles » PMID: 16603619

Rapid, Field-adapted Indirect Enzyme-linked Immunosorbent Assay for Detection of Antibodies in Bovine Whole Blood and Serum to Brucella Abortus

Overview
Journal Clin Vaccine Immunol
Publisher American Society for Microbiology
Specialties Allergy & Immunology
Pathology
Date 2006 Apr 11
PMID 16603619
Citations 1
Authors
D Gall
K Nielsen
W Yu
P Smith
Affiliations
Soon will be listed here.
Abstract

A simple, rapid, field-adapted indirect enzyme-linked immunoassay (FldELISA) for the detection of antibodies to Brucella abortus in whole blood and serum has been developed. This assay detects antibodies to B. abortus in approximately 15 min or less. Over a 3-month period, this assay has consistently identified immunized and nonimmunized animals, while the percent coefficient of variation for each immunized animal has been less than 20%. As with any indirect enzyme-linked immunoassay, quality control can be established and maintained. Using defined positive and negative sera, the sensitivity and specificity of the FldELISA was 100% and 94.2%, respectively. As a model, this test can be readily extended to other disease applications that use lipopolysaccharide or other stable antigens for the detection of antibodies, such as those to Salmonella spp., Escherichia coli, or Yersinia spp.

Citing Articles

Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine.

Bandyopadhyay S, Sasmal D, Dutta T, Ghosh M, Sarkar M, Sasmal N Trop Anim Health Prod. 2008; 41(4):587-92.

PMID: 18763048 DOI: 10.1007/s11250-008-9228-0.

References
1.
Nielsen K, Ballinger R, Stiller J, Rosenbaum B . A "dipstick" enzyme immunoassay for detection of antibody to Brucella abortus in cattle sera. Can J Comp Med. 1985; 49(3):298-302. PMC: 1236174. View
2.
Nielsen K, Gall D, Smith P, Kelly W, Yeo J, Kenny K . Fluorescence polarization assay for the diagnosis of bovine brucellosis: adaptation to field use. Vet Microbiol. 2001; 80(2):163-70. DOI: 10.1016/s0378-1135(00)00386-2. View
3.
Nielsen K, Smith P, Yu W, Nicoletti P, Elzer P, Vigliocco A . Enzyme immunoassay for the diagnosis of brucellosis: chimeric Protein A-Protein G as a common enzyme labeled detection reagent for sera for different animal species. Vet Microbiol. 2004; 101(2):123-9. DOI: 10.1016/j.vetmic.2004.02.014. View
4.
Smits H, Abdoel T, Solera J, Clavijo E, Diaz R . Immunochromatographic Brucella-specific immunoglobulin M and G lateral flow assays for rapid serodiagnosis of human brucellosis. Clin Diagn Lab Immunol. 2003; 10(6):1141-6. PMC: 262433. DOI: 10.1128/cdli.10.6.1141-1146.2003. View
5.
Angus R, Barton C . The production and evaluation of a buffered plate antigen for use in a presumptive test for brucellosis. Dev Biol Stand. 1984; 56:349-56. View