The Structure of the 2[4Fe-4S] Ferredoxin from Pseudomonas Aeruginosa at 1.32-A Resolution: Comparison with Other High-resolution Structures of Ferredoxins and Contributing Structural Features to Reduction Potential Values

Overview

Journal J Biol Inorg Chem

Publisher Springer

Specialty Biochemistry

Date 2006 Apr 6

PMID 16596388

Citations 15

Authors

Petros Giastas

Nikos Pinotsis

Georgios Efthymiou

Matthias Wilmanns

Panayotis Kyritsis

Jean-Marc Moulis

Irene M Mavridis

Affiliations

Soon will be listed here.

Abstract

The structure of the 2[4Fe-4S] ferredoxin (PaFd) from Pseudomonas aeruginosa, which belongs to the Allochromatium vinosum (Alvin) subfamily, has been determined by X-ray crystallography at 1.32-A resolution, which is the highest up to now for a member of this subfamily of Fds. The main structural features of PaFd are similar to those of AlvinFd. However, the significantly higher resolution of the PaFd structure makes possible a reliable comparison with available high-resolution structures of [4Fe-4S]-containing Fds, in an effort to rationalize the unusual electrochemical properties of Alvin-like Fds. Three major factors contributing to the reduction potential values of [4Fe-4S]2+/+ clusters of Fds, namely, the surface accessibility of the clusters, the N-H...S hydrogen-bonding network, and the volume of the cavities hosting the clusters, are extensively discussed. The volume of the cavities is introduced in the present work for the first time, and can in part explain the very negative potential of cluster I of Alvin-like Fds.

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