Photoaffinity Labeling of Indole-3-acetic Acid-binding Proteins in Maize

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1989 Aug 1

PMID 16594060

Citations 27

Authors

A M Jones

M A Venis

Affiliations

Soon will be listed here.

Abstract

The photoaffinity labeling agent 5-azidoindole-3-acetic acid, an analog of the endogenous plant hormone indole-3-acetic acid (an auxin), was used to identify indole-3-acetic acid-binding proteins in maize. Two peptides with subunit molecular masses of 24 and 22 kilodaltons are specifically labeled in a saturable manner. Both peptides are slightly acidic and behave as dimers under nondenaturing conditions. The possibility that one of these peptides is the auxin receptor that mediates cell elongation in maize is discussed.

Citing Articles

Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders in .

Van Fossen E, Kroll J, Anderson L, McNaughton A, Herrera D, Oda Y Appl Environ Microbiol. 2024; 90(10):e0102624.

PMID: 39248464 PMC: 11497774. DOI: 10.1128/aem.01026-24.

Auxin regulation on crop: from mechanisms to opportunities in soybean breeding.

Li L, Chen X Mol Breed. 2023; 43(3):16.

PMID: 37313296 PMC: 10248601. DOI: 10.1007/s11032-023-01361-9.

What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo.

Parizkova B, Pernisova M, Novak O Int J Mol Sci. 2017; 18(12).

PMID: 29258197 PMC: 5751337. DOI: 10.3390/ijms18122736.

Auxin perception and downstream events.

Strader L, Zhao Y Curr Opin Plant Biol. 2016; 33:8-14.

PMID: 27131035 PMC: 5050066. DOI: 10.1016/j.pbi.2016.04.004.

Revisiting Apoplastic Auxin Signaling Mediated by AUXIN BINDING PROTEIN 1.

Feng M, Kim J Mol Cells. 2015; 38(10):829-35.

PMID: 26467289 PMC: 4625063. DOI: 10.14348/molcells.2015.0205.

References

Barbier-Brygoo H, Ephritikhine G, Klambt D, Ghislain M, Guern J . Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. Proc Natl Acad Sci U S A. 1989; 86(3):891-5. PMC: 286584. DOI: 10.1073/pnas.86.3.891. View

Walton J, Ray P . Evidence for Receptor Function of Auxin Binding Sites in Maize : RED LIGHT INHIBITION OF MESOCOTYL ELONGATION AND AUXIN BINDING. Plant Physiol. 1981; 68(6):1334-8. PMC: 426098. DOI: 10.1104/pp.68.6.1334. View

Melhado L, Jones A, LEONARD N, Vanderhoef L . Azido auxins: synthesis and biological activity of fluorescent photoaffinity labeling agents. Plant Physiol. 1981; 68(2):469-75. PMC: 427513. DOI: 10.1104/pp.68.2.469. View

Jones A, Melhado L, Ho T, LEONARD N . Azido auxins: quantitative binding data in maize. Plant Physiol. 1984; 74(2):295-301. PMC: 1066672. DOI: 10.1104/pp.74.2.295. View

Jones A, Melhado L, Ho T, Pearce C, LEONARD N . Azido auxins : photoaffinity labeling of auxin-binding proteins in maize coleoptile with tritiated 5-azidoindole-3-acetic Acid. Plant Physiol. 1984; 75(4):1111-6. PMC: 1067062. DOI: 10.1104/pp.75.4.1111. View

Ray P . Specificity of Auxin-binding Sites on Maize Coleoptile Membranes as Possible Receptor Sites for Auxin Action. Plant Physiol. 1977; 60(4):585-91. PMC: 542669. DOI: 10.1104/pp.60.4.585. View

Hicks G, Rayle D, Jones A, Lomax T . Specific photoaffinity labeling of two plasma membrane polypeptides with an azido auxin. Proc Natl Acad Sci U S A. 1989; 86:4948-52. PMC: 297533. DOI: 10.1073/pnas.86.13.4948. View

Brinegar A, Cooper G, Stevens A, Hauer C, Shabanowitz J, Hunt D . Characterization of a benzyladenine binding-site peptide isolated from a wheat cytokinin-binding protein: sequence analysis and identification of a single affinity-labeled histidine residue by mass spectrometry. Proc Natl Acad Sci U S A. 1988; 85(16):5927-31. PMC: 281878. DOI: 10.1073/pnas.85.16.5927. View

Shimomura S, Sotobayashi T, Futai M, Fukui T . Purification and properties of an auxin-binding protein from maize shoot membranes. J Biochem. 1986; 99(5):1513-24. DOI: 10.1093/oxfordjournals.jbchem.a135621. View

10.

Lobler M, Klambt D . Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). II. Localization of a putative auxin receptor. J Biol Chem. 1985; 260(17):9854-9. View

11.

Lobler M, Klambt D . Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). I. Purification by immunological methods and characterization. J Biol Chem. 1985; 260(17):9848-53. View

12.

Chowdhry V, WESTHEIMER F . Photoaffinity labeling of biological systems. Annu Rev Biochem. 1979; 48:293-325. DOI: 10.1146/annurev.bi.48.070179.001453. View

13.

Ruoho A, Kiefer H, Roeder P, Singer S . The mechanism of photoaffinity labeling. Proc Natl Acad Sci U S A. 1973; 70(9):2567-71. PMC: 427057. DOI: 10.1073/pnas.70.9.2567. View

14.

Ofarrell P . High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975; 250(10):4007-21. PMC: 2874754. View

15.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View