Analysis of Cis-active Sequences Involved in the Leaf-specific Expression of a Potato Gene in Transgenic Plants

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1987 Nov 1

PMID 16593893

Citations 25

Authors

J Stockhaus

P Eckes

M Rocha-Sosa

J Schell

L Willmitzer

Affiliations

Soon will be listed here.

Abstract

ST-LS1 is a light-inducible, single-copy gene from potato that is expressed only in photosynthetic tissues. Various sequences derived from the 5'-upstream region of this gene were fused to the coding region of the chloramphenicol acetyltransferase (CAT) gene and to the gene 7 termination region of the transfer DNA (T-DNA) from the Agrobacterium Ti plasmid pTiACH5 and transferred to tobacco using Ti-plasmid vectors. After regeneration of whole plants, tissues were assayed for the expression of the CAT gene. Sequences derived from the 5'-upstream region of the ST-LS1 gene comprising positions -334 to +11 were sufficient to confer a leaf/stem-specific as well as a light-inducible expression of the CAT gene. Destruction of chloroplasts by treatment with the herbicide norfluorazon and subsequent exposure to light drastically reduced the expression of the CAT gene indicating that this upstream sequence most likely interacts with a chloroplast-dependent signal. When sequences from position -98 to position +675 were fused to a truncated inactive fragment of the cauliflower mosaic virus 35S promoter in a head-to-head manner, the corresponding chimeric genes were again expressed in photosynthetic tissues only, indicating that these sequences have enhancer-like properties.

Citing Articles

Future-Proofing Potato for Drought and Heat Tolerance by Overexpression of Hexokinase and SP6A.

Lehretz G, Sonnewald S, Lugassi N, Granot D, Sonnewald U Front Plant Sci. 2021; 11:614534.

PMID: 33510758 PMC: 7835534. DOI: 10.3389/fpls.2020.614534.

Light regulated transcription in higher plants.

Hiratsuka K, Chua N J Plant Res. 2016; 110(1):131-9.

PMID: 27520053 DOI: 10.1007/BF02506852.

Both developmental and metabolic signals activate the promoter of a class I patatin gene.

Rocha-Sosa M, Sonnewald U, Frommer W, Stratmann M, Schell J, Willmitzer L EMBO J. 1989; 8(1):23-9.

PMID: 16453867 PMC: 400768. DOI: 10.1002/j.1460-2075.1989.tb03344.x.

Anaerobic induction of the maize GapC4 promoter in poplar leaves requires light and high CO2.

Hansch R, Kurz T, Schulze J, Mendel R, Cerff R, Hehl R Planta. 2003; 218(1):79-86.

PMID: 12883886 DOI: 10.1007/s00425-003-1074-8.

The PR-1a promoter contains a number of elements that bind GT-1-like nuclear factors with different affinity.

Buchel A, Molenkamp R, Bol J, Linthorst H Plant Mol Biol. 1996; 30(3):493-504.

PMID: 8605301 DOI: 10.1007/BF00049327.

References

Fluhr R, Kuhlemeier C, Nagy F, Chua N . Organ-specific and light-induced expression of plant genes. Science. 1986; 232(4754):1106-12. DOI: 10.1126/science.232.4754.1106. View

Simpson J, Van Montagu M, Herrera-Estrella L . Photosynthesis-associated gene families: differences in response to tissue-specific and environmental factors. Science. 1986; 233(4759):34-8. DOI: 10.1126/science.233.4759.34. View

Velten J, Schell J . Selection-expression plasmid vectors for use in genetic transformation of higher plants. Nucleic Acids Res. 1985; 13(19):6981-98. PMC: 322017. DOI: 10.1093/nar/13.19.6981. View

Kamalay J, Goldberg R . Regulation of structural gene expression in tobacco. Cell. 1980; 19(4):935-46. DOI: 10.1016/0092-8674(80)90085-9. View

Stockhaus J, Eckes P, Blau A, Schell J, Willmitzer L . Organ-specific and dosage-dependent expression of a leaf/stem specific gene from potato after tagging and transfer into potato and tobacco plants. Nucleic Acids Res. 1987; 15(8):3479-91. PMC: 340743. DOI: 10.1093/nar/15.8.3479. View

Sanchez-Serrano J, Keil M, OConnor A, Schell J, Willmitzer L . Wound expression of a potato proteinase inhibitor II gene in transgenic tobacco plants. EMBO J. 1987; 6(2):303-6. PMC: 553395. DOI: 10.1002/j.1460-2075.1987.tb04754.x. View

Kaulen H, Schell J, Kreuzaler F . Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells. EMBO J. 1986; 5(1):1-8. PMC: 1166687. DOI: 10.1002/j.1460-2075.1986.tb04169.x. View

Timko M, Kausch A, Castresana C, FASSLER J, Herrera-Estrella L, Van den Broeck G . Light regulation of plant gene expression by an upstream enhancer-like element. Nature. 1985; 318(6046):579-82. DOI: 10.1038/318579a0. View

Herrera-Estrella L, Van den Broeck G, Maenhaut R, Van Montagu M, Schell J, Timko M . Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature. 1984; 310(5973):115-20. DOI: 10.1038/310115a0. View

10.

Chen Z, Schuler M, Beachy R . Functional analysis of regulatory elements in a plant embryo-specific gene. Proc Natl Acad Sci U S A. 1986; 83(22):8560-4. PMC: 386970. DOI: 10.1073/pnas.83.22.8560. View

11.

Dean C, Elzen P, Tamaki S, Dunsmuir P, Bedbrook J . Differential expression of the eight genes of the petunia ribulose bisphosphate carboxylase small subunit multi-gene family. EMBO J. 1985; 4(12):3055-61. PMC: 554622. DOI: 10.1002/j.1460-2075.1985.tb04045.x. View

12.

Kamalay J, Goldberg R . Organ-specific nuclear RNAs in tobacco. Proc Natl Acad Sci U S A. 1984; 81(9):2801-5. PMC: 345158. DOI: 10.1073/pnas.81.9.2801. View

13.

Gorman C, Moffat L, Howard B . Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol Cell Biol. 1982; 2(9):1044-51. PMC: 369897. DOI: 10.1128/mcb.2.9.1044-1051.1982. View

14.

Dunsmuir P . The petunia chlorophyll a/b binding protein genes: a comparison of Cab genes from different gene families. Nucleic Acids Res. 1985; 13(7):2503-18. PMC: 341171. DOI: 10.1093/nar/13.7.2503. View