Nucleotide Sequences of the Pseudomonas Savastanoi Indoleacetic Acid Genes Show Homology with Agrobacterium Tumefaciens T-DNA

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Oct 1

PMID 16593610

Citations 62

Authors

T Yamada

C J Palm

B Brooks

T Kosuge

Affiliations

Soon will be listed here.

Abstract

We report the nucleotide sequences of iaaM and iaaH, the genetic determinants for, respectively, tryptophan 2-monooxygenase and indoleacetamide hydrolase, the enzymes that catalyze the conversion of L-tryptophan to indoleacetic acid in the tumor-forming bacterium Pseudomonas syringae pv. savastanoi. The sequence analysis indicates that the iaaM locus contains an open reading frame encoding 557 amino acids that would comprise a protein with a molecular weight of 61,783; the iaaH locus contains an open reading frame of 455 amino acids that would comprise a protein with a molecular weight of 48,515. Significant amino acid sequence homology was found between the predicted sequence of the tryptophan monooxygenase of P. savastanoi and the deduced product of the T-DNA tms-1 gene of the octopine-type plasmid pTiA6NC from Agrobacterium tumefaciens. Strong homology was found in the 25 amino acid sequence in the putative FAD-binding region of tryptophan monooxygenase. Homology was also found in the amino acid sequences representing the central regions of the putative products of iaaH and tms-2 T-DNA. The results suggest a strong similarity in the pathways for indoleacetic acid synthesis encoded by genes in P. savastanoi and in A. tumefaciens T-DNA.

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References

Thomashow L, Reeves S, Thomashow M . Crown gall oncogenesis: evidence that a T-DNA gene from the Agrobacterium Ti plasmid pTiA6 encodes an enzyme that catalyzes synthesis of indoleacetic acid. Proc Natl Acad Sci U S A. 1984; 81(16):5071-5. PMC: 391639. DOI: 10.1073/pnas.81.16.5071. View

Comai L, Kosuge T . Cloning characterization of iaaM, a virulence determinant of Pseudomonas savastanoi. J Bacteriol. 1982; 149(1):40-6. PMC: 216589. DOI: 10.1128/jb.149.1.40-46.1982. View

Henikoff S . Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984; 28(3):351-9. DOI: 10.1016/0378-1119(84)90153-7. View

Comai L, Kosuge T . Transposable element that causes mutations in a plant pathogenic Pseudomonas sp. J Bacteriol. 1983; 154(3):1162-7. PMC: 217587. DOI: 10.1128/jb.154.3.1162-1167.1983. View

Wierenga R, de Jong R, Kalk K, Hol W, Drenth J . Crystal structure of p-hydroxybenzoate hydroxylase. J Mol Biol. 1979; 131(1):55-73. DOI: 10.1016/0022-2836(79)90301-2. View

Vieira J, Messing J . The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982; 19(3):259-68. DOI: 10.1016/0378-1119(82)90015-4. View

Messing J, Vieira J . A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene. 1982; 19(3):269-76. DOI: 10.1016/0378-1119(82)90016-6. View

Rosenberg M, Court D . Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979; 13:319-53. DOI: 10.1146/annurev.ge.13.120179.001535. View

Bassel B, Mills D . Initiation of translation with Pseudomonas aeruginosa phage PP7 RNA: nucleotide sequence of the coat cistron ribosome binding site. Nucleic Acids Res. 1979; 6(5):2003-16. PMC: 327827. DOI: 10.1093/nar/6.5.2003. View

10.

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

11.

Shine J, Dalgarno L . Determinant of cistron specificity in bacterial ribosomes. Nature. 1975; 254(5495):34-8. DOI: 10.1038/254034a0. View

12.

Chilton M, Drummond M, Merio D, Sciaky D, Montoya A, Gordon M . Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell. 1977; 11(2):263-71. DOI: 10.1016/0092-8674(77)90043-5. View

13.

Hutcheson S, Kosuge T . Regulation of 3-indoleacetic acid production in Pseudomonas syringae pv. savastanoi. Purification and properties of tryptophan 2-monooxygenase. J Biol Chem. 1985; 260(10):6281-7. View

14.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

15.

Kosuge T, Heskett M, Wilson E . Microbial synthesis and degradation of indole-3-acetic acid. I. The conversion of L-tryptophan to indole-3-acetamide by an enzyme system from Pseudomonas savastanoi. J Biol Chem. 1966; 241(16):3738-44. View

16.

Messing J . New M13 vectors for cloning. Methods Enzymol. 1983; 101:20-78. DOI: 10.1016/0076-6879(83)01005-8. View

17.

Chen H, Zubay G . Prokaryotic coupled transcription-translation. Methods Enzymol. 1983; 101:674-90. DOI: 10.1016/0076-6879(83)01047-2. View

18.

Inouye S, Ebina Y, Nakazawa A, Nakazawa T . Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida. Proc Natl Acad Sci U S A. 1984; 81(6):1688-91. PMC: 344983. DOI: 10.1073/pnas.81.6.1688. View

19.

Gielen J, De Beuckeleer M, Seurinck J, Deboeck F, De Greve H, Lemmers M . The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5. EMBO J. 1984; 3(4):835-46. PMC: 557436. DOI: 10.1002/j.1460-2075.1984.tb01894.x. View

20.

Schroder G, Waffenschmidt S, Weiler E, Schroder J . The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur J Biochem. 1984; 138(2):387-91. DOI: 10.1111/j.1432-1033.1984.tb07927.x. View