Sequence Determination of the Capsid Protein Gene and Flanking Regions of Tobacco Etch Virus: Evidence for Synthesis and Processing of a Polyprotein in Potyvirus Genome Expression

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1985 Jun 1

PMID 16593574

Citations 22

Authors

R F Allison

J C Sorenson

M E Kelly

F B Armstrong

W G Dougherty

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence of the 3'-terminal portion of the tobacco etch virus (TEV) genome was determined. The 2324-nucleotide sequence represented approximately one-fourth of the TEV genome and included the capsid protein gene and flanking regions. An open reading frame of 2135 nucleotides and an untranslated region of 189 nucleotides adjacent to a polyadenylate tract were identified. The sequence began within an open reading frame, indicating that the initiation codon was upstream of the available sequence data. The sequence of the 20 NH(2)-terminal amino acids of the TEV capsid protein was established chemically. An identical amino acid sequence, predicted from the nucleotide sequence, was located, commencing at amino acid - 263. These data indicated that maturation of the capsid protein required a post-translational cleavage of a larger protein precursor, with a probable cleavage site between the amino acids glutamine and glycine.

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