Chloroplast DNA Transcripts Are Encapsidated by Tobacco Mosaic Virus Coat Protein

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Mar 1

PMID 16593435

Citations 13

Authors

D Rochon

A Siegel

Affiliations

Soon will be listed here.

Abstract

Preparations of tobacco mosaic virus contain pseudovirions, particles resembling virions but containing host rather than viral RNA. The encapsidated host RNA was found to be composed of discrete-sized species derived from a large portion of the chloroplast genome except that very little, if any, ribosomal RNA is present. Pseudovirions contain the same chloroplast DNA transcripts as those detected in extracts from uninfected leaves, although not always in the same relative amounts. Several strains of tobacco mosaic virus were tested and all were found to contain pseudovirions, with the U2 strain containing more than the others.

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Encapsidation of Host RNAs by Cucumber Necrosis Virus Coat Protein during both Agroinfiltration and Infection.

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References

Taylor J, Illmensee R, Summers J . Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976; 442(3):324-30. DOI: 10.1016/0005-2787(76)90307-5. View

Wahl G, Stern M, Stark G . Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proc Natl Acad Sci U S A. 1979; 76(8):3683-7. PMC: 383897. DOI: 10.1073/pnas.76.8.3683. View

Rigby P, Dieckmann M, Rhodes C, Berg P . Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977; 113(1):237-51. DOI: 10.1016/0022-2836(77)90052-3. View

Higgins T, Goodwin P, Whitfeld P . Occurrence of short particles in beans infected with the cowpea strain of TMV. II. Evidence that short particles contain the cistron for coat-protein. Virology. 1976; 71(2):486-97. DOI: 10.1016/0042-6822(76)90376-7. View

Bruening G, Beachy R, Scalla R, Zaitlin M . In vitro and in vivo translation of the ribonucleic acids of a cowpea strain of tobacco mosaic virus. Virology. 1976; 71(2):498-517. DOI: 10.1016/0042-6822(76)90377-9. View

Siegel A, Hari V, Montgomery I, Kolacz K . A messenger RNA for capsid protein isolated from tobacco mosaic virus-infected tissue. Virology. 1976; 73(2):363-71. DOI: 10.1016/0042-6822(76)90397-4. View

Shalla T, Petersen L, Giunchedi L . Partial characterization of virus-like particles in chloroplasts of plants infected with the U5 strain of TMV. Virology. 1975; 66(1):94-105. DOI: 10.1016/0042-6822(75)90181-6. View

BAILEY J, Davidson N . Methylmercury as a reversible denaturing agent for agarose gel electrophoresis. Anal Biochem. 1976; 70(1):75-85. DOI: 10.1016/s0003-2697(76)80049-8. View

Hunter T, Hunt T, Knowland J, Zimmern D . Messenger RNA for the coat protein of tobacco mosaic virus. Nature. 1976; 260(5554):759-64. DOI: 10.1038/260759a0. View

10.

Sippel A . Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli. Eur J Biochem. 1973; 37(1):31-40. DOI: 10.1111/j.1432-1033.1973.tb02953.x. View

11.

Siegel A . Pseudovirions of tobacco mosaic virus. Virology. 1971; 46(1):50-9. DOI: 10.1016/0042-6822(71)90005-5. View

12.

Brakke M, van Pelt N . Linear-log sucrose gradients for estimating sedimentation coefficients of plant viruses and nucleic acids. Anal Biochem. 1970; 38(1):56-64. DOI: 10.1016/0003-2697(70)90155-7. View

13.

Land H, Grez M, Hauser H, Lindenmaier W, Schutz G . 5'-Terminal sequences of eucaryotic mRNA can be cloned with high efficiency. Nucleic Acids Res. 1981; 9(10):2251-66. PMC: 326843. DOI: 10.1093/nar/9.10.2251. View

14.

Ish-Horowicz D, Burke J . Rapid and efficient cosmid cloning. Nucleic Acids Res. 1981; 9(13):2989-98. PMC: 327326. DOI: 10.1093/nar/9.13.2989. View

15.

Grossman A, Bartlett S, Schmidt G, Mullet J, Chua N . Optimal conditions for post-translational uptake of proteins by isolated chloroplasts. In vitro synthesis and transport of plastocyanin, ferredoxin-NADP+ oxidoreductase, and fructose-1,6-bisphosphatase. J Biol Chem. 1982; 257(3):1558-63. View

16.

Palmer J, Edwards H, Jorgensen R, Thompson W . Novel evolutionary variation in transcription and location of two chloroplast genes. Nucleic Acids Res. 1982; 10(21):6819-32. PMC: 326967. DOI: 10.1093/nar/10.21.6819. View

17.

Nishioka Y, Leder A, Leder P . Unusual alpha-globin-like gene that has cleanly lost both globin intervening sequences. Proc Natl Acad Sci U S A. 1980; 77(5):2806-9. PMC: 349493. DOI: 10.1073/pnas.77.5.2806. View

18.

Meshi T, Ohno T, Iba H, Okada Y . Nucleotide sequence of a cloned cDNA copy of TMV (cowpea strain) RNA, including the assembly origin, the coat protein cistron, and the 3' non-coding region. Mol Gen Genet. 1981; 184(1):20-5. DOI: 10.1007/BF00271189. View

19.

Goelet P, Karn J . Tobacco mosaic virus induces the synthesis of a family of 3' coterminal messenger RNAs and their complements. J Mol Biol. 1982; 154(3):541-50. DOI: 10.1016/s0022-2836(82)80013-2. View

20.

Klug A . The assembly of tobacco mosaic virus: structure and specificity. Harvey Lect. 1980; 74:141-72. View