Rapid Degradation of Unassembled Ribulose 1,5-bisphosphate Carboxylase Small Subunits in Chloroplasts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 May 1

PMID 16593310

Citations 112

Authors

G W Schmidt

M L Mishkind

Affiliations

Soon will be listed here.

Abstract

We have detected a proteolytic mechanism in chloroplasts that selectively and rapidly degrades the imported small subunit of ribulose 1,5-bisphosphate carboxylase when pools of the chloroplast-synthesized large subunit are depleted. This degradation system is constitutively present and appears to be responsible for precise stoichiometric accumulation of the two subunits of the enzyme. We believe similar proteolytic mechanisms participate in regulating the accumulation of other photosynthetic proteins during chloroplast biogenesis.

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References

Chua N, Schmidt G . Post-translational transport into intact chloroplasts of a precursor to the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978; 75(12):6110-4. PMC: 393128. DOI: 10.1073/pnas.75.12.6110. View

Hurt E, Hauska G . A cytochrome f/b6 complex of five polypeptides with plastoquinol-plastocyanin-oxidoreductase activity from spinach chloroplasts. Eur J Biochem. 1981; 117(3):591-5. DOI: 10.1111/j.1432-1033.1981.tb06379.x. View

Burke J, Steinback K, Arntzen C . Analysis of the Light-harvesting Pigment-Protein Complex of Wild Type and a Chlorophyll-b-less Mutant of Barley. Plant Physiol. 1979; 63(2):237-43. PMC: 542805. DOI: 10.1104/pp.63.2.237. View

Kirk D, Kirk M . Carrier-mediated Uptake of Arginine and Urea by Chlamydomonas reinhardtii. Plant Physiol. 1978; 61(4):556-60. PMC: 1091916. DOI: 10.1104/pp.61.4.556. View

Weinbaum S, Gressel J, Reisfeld A, Edelman M . Characterization of the 32,000 Dalton Chloroplast Membrane Protein: III. Probing Its Biological Function in Spirodela. Plant Physiol. 1979; 64(5):828-32. PMC: 543372. DOI: 10.1104/pp.64.5.828. View

Schmidt G, Bartlett S, Grossman A, Cashmore A, Chua N . Biosynthetic pathways of two polypeptide subunits of the light-harvesting chlorophyll a/b protein complex. J Cell Biol. 1981; 91(2 Pt 1):468-78. PMC: 2111963. DOI: 10.1083/jcb.91.2.468. View

Desautels M, GOLDBERG A . Demonstration of an ATP-dependent, vanadate-sensitive endoprotease in the matrix of rat liver mitochondria. J Biol Chem. 1982; 257(19):11673-9. View

Bellemare G, Bartlett S, Chua N . Biosynthesis of chlorophyll a/b-binding polypeptides in wild type and the chlorina f2 mutant of barley. J Biol Chem. 1982; 257(13):7762-7. View

Hoober J, Marks D, Keller B, Margulies M . Regulation of accumulation of the major thylakoid polypeptides in Chlamydomonas reinhardtii y-1 at 25 degrees C and 38 degrees C. J Cell Biol. 1982; 95(2 Pt 1):552-8. PMC: 2112964. DOI: 10.1083/jcb.95.2.552. View

10.

Dobberstein B, Blobel G, Chua N . In vitro synthesis and processing of a putative precursor for the small subunit of ribulose-1,5-bisphosphate carboxylase of Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A. 1977; 74(3):1082-5. PMC: 430598. DOI: 10.1073/pnas.74.3.1082. View

11.

Schmidt G, Devillers-Thiery A, Desruisseaux H, Blobel G, Chua N . NH2-terminal amino acid sequences of precursor and mature forms of the ribulose-1,5-bisphosphate carboxylase small subunit from Chlamydomonas reinhardtii. J Cell Biol. 1979; 83(3):615-22. PMC: 2110513. DOI: 10.1083/jcb.83.3.615. View

12.

Feierabend J, Wildner G . Formation of the small subunit in the absence of the large subunit of ribulose 1,5-bisphosphate carboxylase in 70 S ribosome-deficient rye leaves. Arch Biochem Biophys. 1978; 186(2):283-91. DOI: 10.1016/0003-9861(78)90437-x. View

13.

OFarrell P, Goodman H, Ofarrell P . High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977; 12(4):1133-41. DOI: 10.1016/0092-8674(77)90176-3. View

14.

Apel K, Kloppstech K . The plastid membranes of barley (Hordeum vulgare). Light-induced appearance of mRNA coding for the apoprotein of the light-harvesting chlorophyll a/b protein. Eur J Biochem. 1978; 85(2):581-8. DOI: 10.1111/j.1432-1033.1978.tb12273.x. View

15.

Gorman D, Levine R . Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi. Proc Natl Acad Sci U S A. 1965; 54(6):1665-9. PMC: 300531. DOI: 10.1073/pnas.54.6.1665. View

16.

Harris E, Boynton J, Gillham N . Chloroplast ribosome biogenesis in Chlamydomonas. Selection and characterization of mutants blocked in ribosome formation. J Cell Biol. 1974; 63(1):160-79. PMC: 2109351. DOI: 10.1083/jcb.63.1.160. View

17.

Boynton J, Gillham N, Chabot J . Chloroplast ribosome deficient mutants in the green alga Chlamydomonas reinhardi and the question of chloroplast ribosome function. J Cell Sci. 1972; 10(2):267-305. DOI: 10.1242/jcs.10.2.267. View

18.

Givan A, Criddle R . Ribulosediphosphate carboxylase from Chlamydomonas reinhardi: purification, properties and its mode of synthesis in the cell. Arch Biochem Biophys. 1972; 149(1):153-63. DOI: 10.1016/0003-9861(72)90309-8. View

19.

Chua N, Bennoun P . Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975; 72(6):2175-9. PMC: 432719. DOI: 10.1073/pnas.72.6.2175. View

20.

Chua N, Matlin K, Bennoun P . A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii. J Cell Biol. 1975; 67(2PT.1):361-77. PMC: 2109600. DOI: 10.1083/jcb.67.2.361. View