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Theory of Measurement of Förster-type Energy Transfer in Macromolecules

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 1983 May 1
PMID 16593305
Citations 19
Authors
B Epe
K G Steinhauser
P Woolley
Affiliations
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Abstract

We describe the theoretical basis of an unconventional method for the determination of the amount of energy transferred between two fluorophores by the Förster mechanism. The method involves an internal comparison made by separation of the fluorophores in situ (i.e., in the optical cell), for example, by means of enzymic digestion; it eliminates several important sources of error and it simplifies calculation while making maximal use of the information contained in the fluorescence spectra. The validity of the method is demonstrated by determination of the known distance between two modifiable sites on the transfer RNA molecule, and its usefulness is exemplified by its application to triangulation of the ribosome of Escherichia coli.

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References
1.
Carmel A, Zur M, Yaron A, Katchalski E . Use of substrates with fluorescent donor and acceptor chromophores for the kinetic assay of hydrolases. FEBS Lett. 1973; 30(1):11-14. DOI: 10.1016/0014-5793(73)80607-6. View
2.
Haas E, Katchalski-Katzir E, Steinberg I . Effect of the orientation of donor and acceptor on the probability of energy transfer involving electronic transitions of mixed polarization. Biochemistry. 1978; 17(23):5064-70. DOI: 10.1021/bi00616a032. View
3.
Steinhauser K, Woolley P, Epe B, Dijk J . Structure of ribosomal protein L6 from Escherichia coli. A fluorescence study. Eur J Biochem. 1982; 127(3):587-95. DOI: 10.1111/j.1432-1033.1982.tb06913.x. View
4.
Robertus J, Ladner J, Finch J, Rhodes D, Brown R, Clark B . Structure of yeast phenylalanine tRNA at 3 A resolution. Nature. 1974; 250(467):546-51. DOI: 10.1038/250546a0. View
5.
Odom Jr O, Robbins D, Lynch J, Kramer G, Hardesty B . Distances between 3' ends of ribosomal ribonucleic acids reassembled into Escherichia coli ribosomes. Biochemistry. 1980; 19(26):5947-54. DOI: 10.1021/bi00567a001. View