Potato Spindle Tuber and Citrus Exocortis Viroids Undergo No Major Sequence Changes During Replication in Two Different Hosts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1978 Feb 1

PMID 16592502

Citations 7

Authors

E Dickson

T O Diener

H D Robertson

Affiliations

Soon will be listed here.

Abstract

Potato spindle tuber viroid and citrus exocortis viroid, each purified from tomato (Lycopersicon esculentum) and from Gynura aurantiaca, were iodinated in vitro with (125)I, digested with ribonuclease T1, and subjected to two-dimensional RNA fingerprinting analysis. With the exception of minor variations, each viroid retained its distinctive fingerprint pattern irrespective of the host species from which it was isolated. We conclude that the nucleotide sequences of these viroids are principally determined by the infecting viroid and not by the host.

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Evidence for a single rolling circle in the replication of potato spindle tuber viroid.

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Longer-than-unit-length viroid minus strands are present in RNA from infected plants.

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Identification of the RNA region transferred from a representative primer, beta-globin mRNA, to influenza mRNA during in vitro transcription.

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Ultraviolet light-induced crosslinking reveals a unique region of local tertiary structure in potato spindle tuber viroid and HeLa 5S RNA.

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References

Semancik J, Magnuson D, WEATHERS L . Potato spindle tuber disease produced by pathogenic RNA from citrus exocortis disease: evidence for the identity of the causal agents. Virology. 1973; 52(1):292-4. DOI: 10.1016/0042-6822(73)90419-4. View

Robertson H, Dickson E, Model P, Prensky W . Application of fingerprinting techniques to iodinated nucleic acids. Proc Natl Acad Sci U S A. 1973; 70(11):3260-4. PMC: 427212. DOI: 10.1073/pnas.70.11.3260. View

Semancik J, Morris T, WEATHERS L . Structure and conformation of low molecular weight pathogenic RNA from exocortis disease. Virology. 1973; 53(2):448-56. DOI: 10.1016/0042-6822(73)90224-9. View

Robertson H, Jeppesen P . Extent of variation in three related bacteriophage RNA molecules. J Mol Biol. 1972; 68(3):417-28. DOI: 10.1016/0022-2836(72)90096-4. View

Semancik J, WEATHERS L . Pathogenic 10 S RNA from exocortis disease recovered from tomato bunchy-top plants similar to potato spindle tuber virus infection. Virology. 1972; 49(2):622-5. DOI: 10.1016/0042-6822(72)90518-1. View

Zaitlin M, Hariharasubramanian V . A gel electrophoretic analysis of proteins from plants infected with tobacco mosaic and potato spindle tuber viruses. Virology. 1972; 47(2):296-305. DOI: 10.1016/0042-6822(72)90265-6. View

Diener T . Potato spindle tuber viroid. 8. Correlation of infectivity with a UV-absorbing component and thermal denaturation properties of the RNA. Virology. 1972; 50(2):606-9. DOI: 10.1016/0042-6822(72)90412-6. View

Commerford S . Iodination of nucleic acids in vitro. Biochemistry. 1971; 10(11):1993-2000. DOI: 10.1021/bi00787a005. View

Brownlee G, SANGER F . Chromatography of 32P-labelled oligonucleotides on thin layers of DEAE-cellulose. Eur J Biochem. 1969; 11(2):395-9. DOI: 10.1111/j.1432-1033.1969.tb00786.x. View

10.

Robertson H, Dickson E . RNA processing and the control of gene expression. Brookhaven Symp Biol. 1975; (26):240-66. View

11.

Prensky W . The radioiodination of RNA and DNA to high specific activities. Methods Cell Biol. 1976; 13:121-52. DOI: 10.1016/s0091-679x(08)61800-2. View

12.

Takahashi T, Diener T . Potato spindle tuber viroid. XIV. Replication in nuclei isolated from infected leaves. Virology. 1975; 64(1):106-14. DOI: 10.1016/0042-6822(75)90083-5. View

13.

Diener T, Smith D . Potato spindle tuber viroid. XIII. Inhibition of replication by actinomycin D. Virology. 1975; 63(2):421-7. DOI: 10.1016/0042-6822(75)90314-1. View

14.

Franklin R . Purification and properties of the replicative intermediate of the RNA bacteriophage R17. Proc Natl Acad Sci U S A. 1966; 55(6):1504-11. PMC: 224351. DOI: 10.1073/pnas.55.6.1504. View

15.

Diener T . Potato spindle tuber virus: a plant virus with properties of a free nucleic acid. 3. Subcellular location of PSTV-RNA and the question of whether virions exist in extracts or in situ. Virology. 1971; 43(1):75-89. DOI: 10.1016/0042-6822(71)90226-1. View

16.

Hadidi A, Jones D, Gillespie D, Wong-Staal F, Diener T . Hybridization of potato spindle tuber viroid to cellular DNA of normal plants. Proc Natl Acad Sci U S A. 1976; 73(7):2453-7. PMC: 430605. DOI: 10.1073/pnas.73.7.2453. View

17.

Owens R, Erbe E, Hadidi A, Steere R, Diener T . Separation and infectivity of circular and linear forms of potato spindle tuber viroid. Proc Natl Acad Sci U S A. 1977; 74(9):3859-63. PMC: 431760. DOI: 10.1073/pnas.74.9.3859. View

18.

Gross H, Domdey H, Sanger H . Comparative oligonucleotide fingerprints of three plant viroids. Nucleic Acids Res. 1977; 4(6):2021-8. PMC: 342539. DOI: 10.1093/nar/4.6.2021. View

19.

Henco K, Riesner D, Sanger H . Conformation of viroids. Nucleic Acids Res. 1977; 4(1):177-94. PMC: 342418. DOI: 10.1093/nar/4.1.177. View

20.

McClements W, Kaesberg P . Size and secondary structure of potato spindle tuber viroid. Virology. 1977; 76(2):477-84. DOI: 10.1016/0042-6822(77)90230-6. View