Computer Simulation of Fluorescence Depolarization Due to Brownian Motion

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1972 Dec 1

PMID 16592039

Citations 4

Authors

S C Harvey

H C Cheung

Affiliations

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Abstract

A computer program has been written to simulate the Brownian motion of rigid fluorescent molecules. The time dependence of the fluorescence polarization anisotropy as generated by this simulation is in agreement with that predicted by the recent theoretical treatment of Belford, Belford, and Weber (Proc. Nat. Acad. Sci. USA (1972) 69, 1392-1393). The program thus serves as a verification of their equation. It is being generalized to cover the case of nonrigid molecules.

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References

Belford G, Belford R, Weber G . Dynamics of fluorescence polarization in macromolecules. Proc Natl Acad Sci U S A. 1972; 69(6):1392-3. PMC: 426709. DOI: 10.1073/pnas.69.6.1392. View

Weber G . Rotational Brownian motion and polarization of the fluorescence of solutions. Adv Protein Chem. 1953; 8:415-59. DOI: 10.1016/s0065-3233(08)60096-0. View