A Short Estrogen-responsive N-terminal Galanin Homologue Found in Rat Brain and Gut with Antiserum Raised Against Rat Galanin(1-16)

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2006 Apr 6

PMID 16586031

Authors

Susanne Hilke

Tomas Hokfelt

Elvar Theodorsson

Affiliations

Soon will be listed here.

Abstract

Galanin-like peptide (GALP) is currently the only known galanin(1-29) homologue. However, three different galanin receptors, of which GalR3 exhibits comparatively low affinity for galanin(1-29), and molecular heterogeneity of immunoreactive galanin are arguments for presence of other endogenous galanin homologues. Since antibodies recognize three-dimensional structures of 3-5 amino acids in a peptide, we raised antibodies in rabbits against galanin(1-16) conjugated to bovine serum albumin, looking for the presence of endogenous N-terminal galanin homologues in rat tissues. The antiserum selected had 7,830 times higher avidity for galanin(1-16) compared to galanin(1-29). A single immunoreactive component with a Stokes radius of about 8 amino acids was found. Immunohistochemistry strongly suggested that this immunoreactivity is localised in the same neurons as galanin(1-29). Furthermore, its concentration was increased in response to estrogen treatment in the same brain regions as galanin(1-29), although not as rapidly. The present results indicate the presence of a novel endogenous N-terminal galanin homologue.

References

Chng J, Christofides N, Anand P, Gibson S, Allen Y, Su H . Distribution of galanin immunoreactivity in the central nervous system and the responses of galanin-containing neuronal pathways to injury. Neuroscience. 1985; 16(2):343-54. DOI: 10.1016/0306-4522(85)90007-7. View

Melander T, Hokfelt T, Rokaeus A, Cuello A, Oertel W, Verhofstad A . Coexistence of galanin-like immunoreactivity with catecholamines, 5-hydroxytryptamine, GABA and neuropeptides in the rat CNS. J Neurosci. 1986; 6(12):3640-54. PMC: 6568661. View

Bauer F, Christofides N, Hacker G, Blank M, Polak J, Bloom S . Distribution of galanin immunoreactivity in the genitourinary tract of man and rat. Peptides. 1986; 7(1):5-10. DOI: 10.1016/0196-9781(86)90052-5. View

Land T, Langel U, Low M, Berthold M, Unden A, Bartfai T . Linear and cyclic N-terminal galanin fragments and analogs as ligands at the hypothalamic galanin receptor. Int J Pept Protein Res. 1991; 38(3):267-72. DOI: 10.1111/j.1399-3011.1991.tb01438.x. View

Langel U, Bartfai T . Chemistry and molecular biology of galanin receptor ligands. Ann N Y Acad Sci. 1999; 863:86-93. DOI: 10.1111/j.1749-6632.1998.tb10686.x. View

Ohtaki T, Kumano S, Ishibashi Y, Ogi K, Matsui H, Harada M . Isolation and cDNA cloning of a novel galanin-like peptide (GALP) from porcine hypothalamus. J Biol Chem. 1999; 274(52):37041-5. DOI: 10.1074/jbc.274.52.37041. View

Platt J, Michael A . Retardation of fading and enhancement of intensity of immunofluorescence by p-phenylenediamine. J Histochem Cytochem. 1983; 31(6):840-2. DOI: 10.1177/31.6.6341464. View

Ryan M, Gundlach A . Localization of preprogalanin messenger RNA in rat brain: identification of transcripts in a subpopulation of cerebellar Purkinje cells. Neuroscience. 1996; 70(3):709-28. DOI: 10.1016/s0306-4522(96)83009-0. View

de Wied D . The neuropeptide story. Geoffrey Harris Lecture, Budapest, Hungary, July 1994. Front Neuroendocrinol. 1997; 18(1):101-13. DOI: 10.1006/frne.1996.0148. View

10.

Xu Z, Shi T, Hokfelt T . Galanin/GMAP- and NPY-like immunoreactivities in locus coeruleus and noradrenergic nerve terminals in the hippocampal formation and cortex with notes on the galanin-R1 and -R2 receptors. J Comp Neurol. 1998; 392(2):227-51. DOI: 10.1002/(sici)1096-9861(19980309)392:2<227::aid-cne6>3.0.co;2-4. View

11.

Burgevin M, Loquet I, Quarteronet D . Cloning, pharmacological characterization, and anatomical distribution of a rat cDNA encoding for a galanin receptor. J Mol Neurosci. 1995; 6(1):33-41. DOI: 10.1007/BF02736757. View

12.

Takatsu Y, Matsumoto H, Ohtaki T, Kumano S, Kitada C, Onda H . Distribution of galanin-like peptide in the rat brain. Endocrinology. 2001; 142(4):1626-34. DOI: 10.1210/endo.142.4.8089. View

13.

Jureus A, Cunningham M, Li D, Johnson L, Krasnow S, Teklemichael D . Distribution and regulation of galanin-like peptide (GALP) in the hypothalamus of the mouse. Endocrinology. 2001; 142(12):5140-4. DOI: 10.1210/endo.142.12.8542. View

14.

Gabriel S, Knott P, Haroutunian V . Alterations in cerebral cortical galanin concentrations following neurotransmitter-specific subcortical lesions in the rat. J Neurosci. 1995; 15(8):5526-34. PMC: 6577642. View

15.

Parker E, Izzarelli D, Nowak H, Mahle C, Iben L, Wang J . Cloning and characterization of the rat GALR1 galanin receptor from Rin14B insulinoma cells. Brain Res Mol Brain Res. 1995; 34(2):179-89. DOI: 10.1016/0169-328x(95)00159-p. View

16.

Xu Z, Ma X, Soomets U, Langel U, Hokfelt T . Electrophysiological evidence for a hyperpolarizing, galanin (1-15)-selective receptor on hippocampal CA3 pyramidal neurons. Proc Natl Acad Sci U S A. 1999; 96(25):14583-7. PMC: 24479. DOI: 10.1073/pnas.96.25.14583. View

17.

Hedlund P, Yanaihara N, Fuxe K . Evidence for specific N-terminal galanin fragment binding sites in the rat brain. Eur J Pharmacol. 1992; 224(2-3):203-5. DOI: 10.1016/0014-2999(92)90806-f. View

18.

Xu X, Wiesenfeld-Hallin Z, Fisone G, Bartfai T, Hokfelt T . The N-terminal 1-16, but not C-terminal 17-29, galanin fragment affects the flexor reflex in rats. Eur J Pharmacol. 1990; 182(1):137-41. DOI: 10.1016/0014-2999(90)90502-w. View

19.

Adams J . Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem. 1992; 40(10):1457-63. DOI: 10.1177/40.10.1527370. View

20.

Gundlach A, Burazin T . Galanin-galanin receptor systems in the hypothalamic paraventricular and supraoptic nuclei. Some recent findings and future challenges. Ann N Y Acad Sci. 1999; 863:241-51. DOI: 10.1111/j.1749-6632.1998.tb10699.x. View