Mutant Glycosyltransferases Assist in the Development of a Targeted Drug Delivery System and Contrast Agents for MRI

Overview

Journal AAPS J

Specialty Pharmacology

Date 2006 Apr 6

PMID 16584127

Citations 5

Authors

Pradman K Qasba

Boopathy Ramakrishnan

Elizabeth Boeggeman

Affiliations

Soon will be listed here.

Abstract

The availability of structural information on glycosyltransferases is beginning to make structure-based reengineering of these enzymes possible. Mutant glycosyltransferases have been generated that can transfer a sugar residue with a chemically reactive unique functional group to a sugar moiety of glycoproteins, glycolipids, and proteoglycans (glycoconjugates). The presence of modified sugar moiety on a glycoprotein makes it possible to link bioactive molecules via modified glycan chains, thereby assisting in the assembly of bionanoparticles that are useful for developing the targeted drug delivery system and contrast agents for magnetic resonance imaging. The reengineered recombinant glycosyltransferases also make it possible to (1) remodel the oligosaccharide chains of glycoprotein drugs, and (2) synthesize oligosaccharides for vaccine development.

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Glycoengineering of Antibodies for Modulating Functions.

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Site-specific linking of biomolecules via glycan residues using glycosyltransferases.

Qasba P, Boeggeman E, Ramakrishnan B Biotechnol Prog. 2008; 24(3):520-6.

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Applications of glycosyltransferases in the site-specific conjugation of biomolecules and the development of a targeted drug delivery system and contrast agents for MRI.

Ramakrishnan B, Boeggeman E, Qasba P Expert Opin Drug Deliv. 2008; 5(2):149-53.

PMID: 18248315 PMC: 2291282. DOI: 10.1517/17425247.5.2.149.

Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method.

Boeggeman E, Ramakrishnan B, Kilgore C, Khidekel N, Hsieh-Wilson L, Simpson J Bioconjug Chem. 2007; 18(3):806-14.

PMID: 17370997 PMC: 3534963. DOI: 10.1021/bc060341n.

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