Development of Aldosterone-stimulation of Short-circuit Current Across Larval Frog Skin

Overview

Journal J Comp Physiol B

Specialties Biochemistry
Endocrinology
Physiology

Date 1991 Jan 1

PMID 1658089

Authors

S D Hillyard

W Van Driessche

Affiliations

Soon will be listed here.

Abstract

The short-circuit current (SCC) across isolated skin from bullfrog larvae in developmental stage XXI was small and insensitive to amiloride. Overnight incubation of this tissue with 10(-6) M aldosterone stimulated the SCC from 1.35 +/- 0.55 to 14.55 +/- 4.12 microA.cm-2 with 11.18 +/- 4.46 microA.cm-2 being blocked by 100 microM amiloride. Histologic examination of aldosterone-treated skins revealed a separation of the apical cell layer from the underlying epidermis that was not seen in untreated preparations. The onset of amiloride-sensitive Na+ transport thus coincided with the exposure of the apical surface of newly differentiated epithelial cells. Similar results were obtained with skin from stage XXI larvae whose rate of metamorphosis had been stimulated by 10 micrograms.1-1 thyroxine (T4) but not with skin from T4-treated larvae in stages XIX and XX. Fluctuation analysis of the amiloride-sensitive SCC of the above preparations failed to show a consistent Lorentzian component in the power-density spectrum. Fluctuation analysis was possible on skins from larvae whose development had been accelerated by 7-9 days treatment with 10 micrograms.l-1 triiodothyronine (T3). Aldosterone treatment of these tissues resulted in a significant increase in Na+ channel density.

References

Takada M . Differentiation of frog skin active Na+ transport during metamorphosis is induced by thyroid hormone. Gen Comp Endocrinol. 1990; 77(3):442-7. DOI: 10.1016/0016-6480(90)90235-e. View

Van Driessche W, Lindemann B . Low-noise amplification of voltage and current fluctuations arising in epithelia. Rev Sci Instrum. 1978; 49(1):52. DOI: 10.1063/1.1135251. View

Robinson D, MILLS J . Ouabain binding in tadpole ventral skin. II. Localization of Na pump sites. Am J Physiol. 1987; 253(3 Pt 2):R410-7. DOI: 10.1152/ajpregu.1987.253.3.R410. View

Kikuyama S, Suzuki M, Iwamuro S . Elevation of plasma aldosterone levels of tadpoles at metamorphic climax. Gen Comp Endocrinol. 1986; 63(2):186-90. DOI: 10.1016/0016-6480(86)90155-3. View

Hillyard S, Zeiske W, Van Driessche W . Poorly selective cation channels in the skin of the larval frog (stage less than or equal to XIX). Pflugers Arch. 1982; 394(4):287-93. DOI: 10.1007/BF00583692. View

Lindemann B, Van Driessche W . Sodium-specific membrane channels of frog skin are pores: current fluctuations reveal high turnover. Science. 1977; 195(4275):292-4. DOI: 10.1126/science.299785. View

Porter G . The action of aldosterone on transepithelial sodium transport in isolated ventral toad skin. Gen Comp Endocrinol. 1971; 16(3):443-51. DOI: 10.1016/0016-6480(71)90108-0. View

Benos D, Mandel L, Balaban R . On the mechanism of the amiloride-sodium entry site interaction in anuran skin epithelia. J Gen Physiol. 1979; 73(3):307-26. PMC: 2215163. DOI: 10.1085/jgp.73.3.307. View

Buron M, Navas P . Thyroxin specifically stimulates anuran larvae epidermal sodium pump during metamorphosis. Comp Biochem Physiol A Comp Physiol. 1988; 91(4):693-5. DOI: 10.1016/0300-9629(88)90951-6. View

10.

Van Driessche W, Zeiske W . Ca2+-sensitive, spontaneously fluctuating, cation channels in the apical membrane of the adult frog skin epithelium. Pflugers Arch. 1985; 405(3):250-9. DOI: 10.1007/BF00582569. View

11.

Taylor A, KOLLROS J . Stages in the normal development of Rana pipiens larvae. Anat Rec. 2010; 94:7-13. DOI: 10.1002/ar.1090940103. View

12.

Abramcheck F, Van Driessche W, Helman S . Autoregulation of apical membrane Na+ permeability of tight epithelia. Noise analysis with amiloride and CGS 4270. J Gen Physiol. 1985; 85(4):555-82. PMC: 2215807. DOI: 10.1085/jgp.85.4.555. View

13.

Robinson D, MILLS J . Ouabain binding in tadpole ventral skin. I. Kinetics and effect on intracellular ions. Am J Physiol. 1987; 253(3 Pt 2):R402-9. DOI: 10.1152/ajpregu.1987.253.3.R402. View

14.

Hillyard S, Van Driessche W . Effect of amiloride on the poorly selective cation channel of larval bullfrog skin. Am J Physiol. 1989; 256(1 Pt 1):C168-74. DOI: 10.1152/ajpcell.1989.256.1.C168. View

15.

De Wolf I, Van Driessche W . Voltage-dependent Ba2+ block of K+ channels in apical membrane of frog skin. Am J Physiol. 1986; 251(5 Pt 1):C696-706. DOI: 10.1152/ajpcell.1986.251.5.C696. View

16.

Brown S, Savage L, Brown P . Electrophysiology of newt skin: responses to salt-depletion, aldosterone, arginine vasotocin and epinephrine. Comp Biochem Physiol A Comp Physiol. 1988; 91(4):715-9. DOI: 10.1016/0300-9629(88)90955-3. View

17.

Hillyard S, Zeiske W, Van Driessche W . A fluctuation analysis study of the development of amiloride-sensitive Na+ transport in the skin of larval bullfrogs (Rana catesbeiana). Biochim Biophys Acta. 1982; 692(3):455-61. DOI: 10.1016/0005-2736(82)90397-2. View

18.

Palmer L, Li J, Lindemann B, Edelman I . Aldosterone control of the density of sodium channels in the toad urinary bladder. J Membr Biol. 1982; 64(1-2):91-102. DOI: 10.1007/BF01870771. View

19.

Cox T, ALVARADO R . Electrical and transport characteristics of skin of larval Rana catesbeiana. Am J Physiol. 1979; 237(1):R74-9. DOI: 10.1152/ajpregu.1979.237.1.R74. View

20.

TAYLOR Jr R, BARKER S . TRANSEPIDERMAL POTENTIAL DIFFERENCE: DEVELOPMENT IN ANURAN LARVAE. Science. 1965; 148(3677):1612-3. DOI: 10.1126/science.148.3677.1612. View