Conservation of Residues Involved in Sugar/H(+) Symport by the Sucrose Permease of Escherichia Coli Relative to Lactose Permease

Overview

Journal J Mol Biol

Publisher Elsevier

Specialties Microbiology
Molecular Biology

Date 2006 Apr 1

PMID 16574149

Citations 20

Authors

Viveka Vadyvaloo

Irina N Smirnova

Vladimir N Kasho

H Ronald Kaback

Affiliations

Soon will be listed here.

Abstract

Building a three-dimensional model of the sucrose permease of Escherichia coli (CscB) with the X-ray crystal structure lactose permease (LacY) as template reveals a similar overall fold for CscB. Moreover, despite only 28% sequence identity and a marked difference in substrate specificity, the structural organization of the residues involved in sugar-binding and H(+) translocation is conserved in CscB. Functional analyses of mutants in the homologous key residues provide strong evidence that they play a similar critical role in the mechanisms of CscB and LacY.

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