Role of Endosomal Cathepsins in Entry Mediated by the Ebola Virus Glycoprotein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2006 Mar 31

PMID 16571833

Citations 279

Authors

Kathryn Schornberg

Shutoku Matsuyama

Kirsten Kabsch

Sue Delos

Amy Bouton

Judith White

Affiliations

Soon will be listed here.

Abstract

Using chemical inhibitors and small interfering RNA (siRNA), we have confirmed roles for cathepsin B (CatB) and cathepsin L (CatL) in Ebola virus glycoprotein (GP)-mediated infection. Treatment of Ebola virus GP pseudovirions with CatB and CatL converts GP1 from a 130-kDa to a 19-kDa species. Virus with 19-kDa GP1 displays significantly enhanced infection and is largely resistant to the effects of the CatB inhibitor and siRNA, but it still requires a low-pH-dependent endosomal/lysosomal function. These and other results support a model in which CatB and CatL prime GP by generating a 19-kDa intermediate that can be acted upon by an as yet unidentified endosomal/lysosomal enzyme to trigger fusion.

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