Proteins Pattern Alteration in AZT-treated K562 Cells Detected by Two-dimensional Gel Electrophoresis and Peptide Mass Fingerprinting

Overview

Journal Proteome Sci

Publisher Biomed Central

Date 2006 Mar 31

PMID 16571109

Citations 3

Authors

Gabriele DAndrea

Anna R Lizzi

Sara Venditti

Laura Di Francesco

Alessandra Giorgi

Giuseppina Mignogna

Arduino Oratore

Argante Bozzi

Affiliations

Soon will be listed here.

Abstract

In this study we report the effect of AZT on the whole protein expression profile both in the control and the AZT-treated K562 cells, evidenced by two-dimensional gel electrophoresis and peptide mass fingerprinting analysis. Two-dimensional gels computer digital image analysis showed two spots that appeared up-regulated in AZT-treated cells and one spot present only in the drug exposed samples. Upon extraction and analysis by peptide mass fingerprinting, the first two spots were identified as PDI-A3 and stathmin, while the third one was proved to be NDPK-A. Conversely, two protein spots were present only in the untreated K562 cells, and were identified as SOD1 and HSP-60, respectively.

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