Molecular Regulation of Apoptosis in Fast Plantaris Muscles of Aged Rats

Overview

Journal J Gerontol A Biol Sci Med Sci

Specialty Geriatrics

Date 2006 Mar 29

PMID 16567372

Citations 36

Authors

Emidio E Pistilli

Parco M Siu

Stephen E Alway

Affiliations

Soon will be listed here.

Abstract

This study tested the hypothesis that aging exacerbates apoptotic signaling in rat fast plantaris muscle during muscle unloading. Plantaris muscle mass was 22% lower in aged animals and the apoptotic index was 600% higher, when compared to those in young adult animals. Following 14 days of hind-limb unloading, absolute plantaris muscle mass was 20% lower in young adult animals with a corresponding 200% higher elevation of the apoptotic index. Unloading had no affect on muscle weight or apoptotic index of aged plantaris muscles. The changes in pro-apoptotic messenger RNA (mRNA) for apoptotic protease activating factor-1 (Apaf-1), Bax, and inhibitor of differentiation protein-2 (Id2) were exacerbated with aging. Bax and Bcl-2 protein levels were also altered differently in aged muscle, compared to young. Significant positive correlations were observed between the changes in Id2 and Bax mRNA, and Id2 and caspase-9 mRNA. These data suggest that a pro-apoptotic environment may contribute to aging-associated atrophy in fast skeletal muscle, but apoptotic signaling differs by age.

Citing Articles

Paez H, Pitzer C, Alway S Cells. 2023; 12(2).

PMID: 36672183 PMC: 9856405. DOI: 10.3390/cells12020249.

MBNL1-Associated Mitochondrial Dysfunction and Apoptosis in C2C12 Myotubes and Mouse Skeletal Muscle.

Yokoyama S, Ohno Y, Egawa T, Ohashi K, Ito R, Ortuste Quiroga H Int J Mol Sci. 2020; 21(17).

PMID: 32887414 PMC: 7503908. DOI: 10.3390/ijms21176376.

The Effects of Calcium--Hydroxy--Methylbutyrate on Aging-Associated Apoptotic Signaling and Muscle Mass and Function in Unloaded but Nonatrophied Extensor Digitorum Longus Muscles of Aged Rats.

Bennett B, Mohamed J, Alway S Oxid Med Cell Longev. 2020; 2020:3938672.

PMID: 32774671 PMC: 7396042. DOI: 10.1155/2020/3938672.

mTORC1 underlies age-related muscle fiber damage and loss by inducing oxidative stress and catabolism.

Tang H, Inoki K, Brooks S, Okazawa H, Lee M, Wang J Aging Cell. 2019; 18(3):e12943.

PMID: 30924297 PMC: 6516169. DOI: 10.1111/acel.12943.

Effects of hindlimb suspension and reloading on gastrocnemius and soleus muscle mass and function in geriatric mice.

Oliveira J, Mohamed J, Myers M, Brooks M, Alway S Exp Gerontol. 2018; 115:19-31.

PMID: 30448397 PMC: 6366863. DOI: 10.1016/j.exger.2018.11.011.

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