Neurovirulence of Six Different Murine Coronavirus JHMV Variants for Rats

Overview

Journal Virus Res

Specialty Microbiology

Date 1991 Jun 1

PMID 1656623

Citations 8

Authors

Y Matsubara

R Watanabe

F Taguchi

Affiliations

Soon will be listed here.

Abstract

Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.

Citing Articles

Spongiform degeneration induced by neuropathogenic murine coronavirus infection.

Kashiwazaki H, Nomura R, Matsuyama S, Taguchi F, Watanabe R Pathol Int. 2011; 61(4):184-91.

PMID: 21418390 PMC: 7167946. DOI: 10.1111/j.1440-1827.2010.02639.x.

Murine coronavirus neuropathogenesis: determinants of virulence.

Cowley T, Weiss S J Neurovirol. 2010; 16(6):427-34.

PMID: 21073281 PMC: 3153983. DOI: 10.3109/13550284.2010.529238.

Communication between S1N330 and a region in S2 of murine coronavirus spike protein is important for virus entry into cells expressing CEACAM1b receptor.

Matsuyama S, Taguchi F Virology. 2002; 295(1):160-71.

PMID: 12033774 PMC: 7133742. DOI: 10.1006/viro.2002.1391.

Identification of spike protein residues of murine coronavirus responsible for receptor-binding activity by use of soluble receptor-resistant mutants.

Saeki K, Ohtsuka N, Taguchi F J Virol. 1997; 71(12):9024-31.

PMID: 9371559 PMC: 230203. DOI: 10.1128/JVI.71.12.9024-9031.1997.

Fusion formation by the uncleaved spike protein of murine coronavirus JHMV variant cl-2.

Taguchi F J Virol. 1993; 67(3):1195-202.

PMID: 8437210 PMC: 237484. DOI: 10.1128/JVI.67.3.1195-1202.1993.

References

Massa P, Dorries R, Ter Meulen V . Viral particles induce Ia antigen expression on astrocytes. Nature. 1986; 320(6062):543-6. PMC: 7094962. DOI: 10.1038/320543a0. View

Taguchi F, Yamada A, Fujiwara K . Resistance to highly virulent mouse hepatitis virus acquired by mice after low-virulence infection: enhanced antiviral activity of macrophages. Infect Immun. 1980; 29(1):42-9. PMC: 551072. DOI: 10.1128/iai.29.1.42-49.1980. View

Koga M, Wege H, Ter Meulen V . Sequence of murine coronavirus JHM induced neuropathological changes in rats. Neuropathol Appl Neurobiol. 1984; 10(3):173-84. PMC: 7168023. DOI: 10.1111/j.1365-2990.1984.tb00350.x. View

Nagashima K, Wege H, Meyermann R, Ter Meulen V . Demyelinating encephalomyelitis induced by a long-term corona virus infection in rats. A preliminary report. Acta Neuropathol. 1979; 45(3):205-13. PMC: 7086537. DOI: 10.1007/BF00702672. View

Wege H, Winter J, Meyermann R . The peplomer protein E2 of coronavirus JHM as a determinant of neurovirulence: definition of critical epitopes by variant analysis. J Gen Virol. 1988; 69 ( Pt 1):87-98. DOI: 10.1099/0022-1317-69-1-87. View

Makino S, Taguchi F, Fujiwara K . Defective interfering particles of mouse hepatitis virus. Virology. 1984; 133(1):9-17. PMC: 7131828. DOI: 10.1016/0042-6822(84)90420-3. View

Dorries R, Wege H . Hybridoma antibodies to the murine coronavirus JHM: characterization of epitopes on the peplomer protein (E2). J Gen Virol. 1984; 65 ( Pt 11):1931-42. DOI: 10.1099/0022-1317-65-11-1931. View

Sorensen O, Dugre R, Percy D, Dales S . In vivo and in vitro models of demyelinating disease: endogenous factors influencing demyelinating disease caused by mouse hepatitis virus in rats and mice. Infect Immun. 1982; 37(3):1248-60. PMC: 347672. DOI: 10.1128/iai.37.3.1248-1260.1982. View

Schmidt I, Skinner M, Siddell S . Nucleotide sequence of the gene encoding the surface projection glycoprotein of coronavirus MHV-JHM. J Gen Virol. 1987; 68 ( Pt 1):47-56. DOI: 10.1099/0022-1317-68-1-47. View

10.

Beushausen S, Dales S . In vivo and in vitro models of demyelinating disease. XI. Tropism and differentiation regulate the infectious process of coronaviruses in primary explants of the rat CNS. Virology. 1985; 141(1):89-101. PMC: 7131688. DOI: 10.1016/0042-6822(85)90185-0. View

11.

Hondo R, Kurata T, Sato S, Oda A, Aoyama Y . Enzymatic treatment of formalin-fixed and paraffin-embedded specimens for detection of antigens of herpes simplex, varicella-zoster and human cytomegaloviruses. Jpn J Exp Med. 1982; 52(1):17-25. View

12.

Watanabe R, Wege H, Ter Meulen V . Comparative analysis of coronavirus JHM-induced demyelinating encephalomyelitis in Lewis and Brown Norway rats. Lab Invest. 1987; 57(4):375-84. View

13.

Koga M, Wege H, Ter Meulen V . JHM infections in rats as a model for acute and subacute demyelinating disease. Adv Exp Med Biol. 1981; 142:327-40. DOI: 10.1007/978-1-4757-0456-3_27. View

14.

Sorensen O, Coulter-Mackie M, Puchalski S, Dales S . In vivo and in vitro models of demyelinating disease. IX. Progression of JHM virus infection in the central nervous system of the rat during overt and asymptomatic phases. Virology. 1984; 137(2):347-57. PMC: 7130679. DOI: 10.1016/0042-6822(84)90227-7. View

15.

Holland J, Spindler K, Horodyski F, Grabau E, Nichol S, VandePol S . Rapid evolution of RNA genomes. Science. 1982; 215(4540):1577-85. DOI: 10.1126/science.7041255. View

16.

Holmes K, Doller E, Behnke J . Analysis of the functions of coronavirus glycoproteins by differential inhibition of synthesis with tunicamycin. Adv Exp Med Biol. 1981; 142:133-42. DOI: 10.1007/978-1-4757-0456-3_11. View

17.

Fleming J, Trousdale M, Bradbury J, Stohlman S, Weiner L . Experimental demyelination induced by coronavirus JHM (MHV-4): molecular identification of a viral determinant of paralytic disease. Microb Pathog. 1987; 3(1):9-20. PMC: 7134751. DOI: 10.1016/0882-4010(87)90033-7. View

18.

Nagashima K, Wege H, Meyermann R, Ter Meulen V . Corona virus induced subacute demyelinating encephalomyelitis in rats: a morphological analysis. Acta Neuropathol. 1978; 44(1):63-70. PMC: 7086507. DOI: 10.1007/BF00691641. View

19.

Taguchi F, Massa P, Ter Meulen V . Characterization of a variant virus isolated from neural cell culture after infection of mouse coronavirus JHMV. Virology. 1986; 155(1):267-70. PMC: 7131492. DOI: 10.1016/0042-6822(86)90187-x. View

20.

Wege H, Watanabe R, Ter Meulen V . Relapsing subacute demyelinating encephalomyelitis in rats during the course of coronavirus JHM infection. J Neuroimmunol. 1984; 6(5):325-36. PMC: 7119698. DOI: 10.1016/0165-5728(84)90022-5. View