An Erythroid Specific Enhancer Upstream to the Gene Encoding the Cell-type Specific Transcription Factor GATA-1

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1991 Oct 11

PMID 1656391

Citations 33

Authors

S Nicolis

C Bertini

A Ronchi

S Crotta

L Lanfranco

E Moroni

B Giglioni

S Ottolenghi

Affiliations

Soon will be listed here.

Abstract

The transcription factor GATA-1 is expressed in a subset of hemopoietic cells, where it mediates the cell-type specific expression of several genes. We have cloned the mouse and human GATA-1 genes. A region upstream to the first exon, and highly conserved between mouse and man, acts as an erythroid specific enhancer in transient assays, if linked to the GATA-1 or to the SV40 promoter. The activity of the enhancer is almost completely dependent on the integrity of a dimeric GATA-1 binding site.

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