Effect of Formalin Treatment on Electrophoretic Mobility of Cholera Toxin
Overview
Overview
Journal
Infect Immun
Publisher
American Society for Microbiology
Specialty
Allergy & Immunology
Date
1970 Jul 1
PMID
16557792
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
Crude cholera toxin detoxified by treatment with Formalin showed an increased mobility and a decrease in the number of detectable protein constituents when compared with the parent toxin by disc electrophoresis. The significance of these observations in relation to the enhanced antigenic activity of cholera toxoid as compared to parent toxin is discussed.
References
1.
Ornstein L
. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964; 121:321-49.
DOI: 10.1111/j.1749-6632.1964.tb14207.x.
View
2.
Finkelstein R, LoSpalluto J
. Pathogenesis of experimental cholera. Preparation and isolation of choleragen and choleragenoid. J Exp Med. 1969; 130(1):185-202.
PMC: 2138674.
DOI: 10.1084/jem.130.1.185.
View
3.
Coleman W, Kaur J, IWERT M, KASAI G, Burrows W
. Cholera toxins: purification and preliminary characterization of ileal loop reactive type 2 toxin. J Bacteriol. 1968; 96(4):1137-43.
PMC: 252427.
DOI: 10.1128/jb.96.4.1137-1143.1968.
View
4.
MURPHY S
. Tetanus toxin and antigenic derivatives. II. Effect of protein and formaldehyde concentration on toxoid formation. J Bacteriol. 1967; 94(3):586-9.
PMC: 251927.
DOI: 10.1128/jb.94.3.586-589.1967.
View
5.
Craig J
. A permeability factor (toxin) found in cholera stools and culture filtrates and its neutralization by convalescent cholera sera. Nature. 1965; 207(997):614-6.
DOI: 10.1038/207614a0.
View